HPLC测定香砂养胃丸中橙皮苷和柚皮苷含量  

Determination of Hesperidin and naringoside in xiangsha yangwei pill by HPLC

作　　者：刘思曼[1] 边清泉[1] 唐英[2] 张进[2] 李方[3] 

机构地区：[1]绵阳师范学院天然产物研究所,四川绵阳621000 [2]重庆文理学院化学与环境科学系,重庆永川402168 [3]四川大学化学学院,四川成都610064

出　　处：《化学研究与应用》2008年第9期1196-1198,共3页Chemical Research and Application

基　　金：绵阳市科技局自然科学研究重点项目(2006S016)

摘　　要：Hesperidin and naringoside in xiangsha yangwei pill has been determined by the HPLC,in which the DiamonsilTMC18(4.6 mm×150 mm,5 μm)was used as the column at UV 283 nm and the CH3CN-H2O solution(22∶78) was made use of mobile phase,in which H3PO4 was used as agent to regulate pH(3.5),the flow rate is 1.2 mL·min-1,there was a fine separated degree between the pending component and other component and the lineal range of Hesperidin and naringoside is 0.25μg^1.49 μg and 0.023μg^0.14 μg respectively.The average recoveries of Hesperidin and Naringoside were 99.3% and 102.6% respectively with RSD being 1.2% and 2.5%.The result can give a simple,effective method for controlling the quality of xiangsha yangwei pill.Hesperidin and naringoside in xiangsha yangwei piU has been determined by the HPLC, in which the DiamonsilTMCis （4. 6 nun × 150 mm,5 μm）was used as the column at UV 283 run and the CH3CN-H2O solution （22：78） was made use of mobile phase,in which H3PO4 was used as agent to regulate pH（3.5 ） ,the flow rate is 1.2 mL · min^-1 ,there was a fine separated degree between the pending omponent and other component and the lineal range of Hesperidin and naringoside is 0. 25μg - 1.49μg and 0. 023μg -0. 14μg respectivdy. The average recoveries of Heaperidin and Naringoside were 99. 3% and 102. 6% respectively with RSD being 1.2% and 2.5% . The result can give a simple,effective method for controlling the quality of xiangsha yangwei pill.

关 键 词：HPLC 香砂养胃丸 橙皮苷 柚皮苷 

分 类 号：O657.7[理学—分析化学]