An EAL domain protein and cyclic AMP contribute to the interaction between the two quorum sensing systems in Escherichia coli  

作　　者：Xianxuan Zhou Xiaoming Meng Baolin Sun 

机构地区：[1]Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China

出　　处：《Cell Research》2008年第9期937-948,共12页细胞研究（英文版）

摘　　要：Quorum sensing （QS） is a bacterial cell-cell communication process by which bacteria communicate using extracellular signals called autoinducers. Two QS systems have been identified in Escherichia coli K-12, including an intact QS system 2 that is stimulated by the cyclic AMP （cAMP）-cAMP receptor protein （CRP） complex and a partial QS system 1 that consists of SdiA （suppressor of cell division inhibitor） responding to signals generated by other microbial species. The relationship between QS system 1 and system 2 in E. coli, however, remains obscure. Here, we show that an EAL domain protein, encoded by ydiV, and cAMP are involved in the interaction between the two QS systems in E. coli. Expression of sdiA and ydiV is inhibited by glucose. SdiA binds to the ydiV promoter region in a dose-dependent, but nonspecific, manner; extracellular autoinducer 1 from other species stimulates ydiV expression in an sdiA-depen- dent manner. Furthermore, we discovered that the double sdiA-ydiV mutation, but not the single mutation, causes a 2-fold decrease in intracellular cAMP concentration that leads to the inhibition of QS system 2. These results indicate that signaling pathways that respond to important environmental cues, such as autoinducers and glucose, are linked together for their control in E. coli.

关 键 词：quorum sensing ydi V CAMP 

分 类 号：Q939.1[生物学—微生物学]