猪MyL4基因的克隆及其在猪胚胎骨骼肌中的表达分析  

Clone of MYL4 Gene and Its Expression Analysis in Pig Embryo Skeletal Muscle

作　　者：伍晓雄[1] 唐中林[2] 邓宏[1] 李勇 储明星[2] 马月辉[2] 李奎[2] 

机构地区：[1]华中农业大学动物遗传育种教育部重点实验室,武汉430070 [2]中国农业科学院家养动物遗传资源与种质创新重点开放实验室,北京100094

出　　处：《农业生物技术学报》2008年第4期580-585,共6页Journal of Agricultural Biotechnology

基　　金：国家高技术研究与发展计划(863)项目(No.20060110Z1039);国家基础研究发展规划(973)项目(No.2006CB102105);欧盟第六框架合作项目(No.036245-2);国家自然科学基金(No.30571300);中国农业科学院北京畜牧兽医研究所科技创新团队项目资助。

摘　　要：克隆了猪(Sus scrofa)MYL4基因的cDNA序列,并利用生物信息学方法进行验证。所得cDNA序列全长1 105 bp,包含1个完整的开放阅读框,编码197个氨基酸。序列分析表明,该基因与已报道的狗(Canis lupus familiaris)、人(Homo sapiens)、黑猩猩(Pan troglodytes)和恒河猴(Macaca mulatta)等物种的MYL4基因高度同源,氨基酸序列同源性分别为98.4%、95.9%、95.4%和95.4%。该基因编码的蛋白具有EFH和FRQ1保守功能域。荧光定量分析该基因在猪胚胎骨骼肌(妊娠33、65和90 d)中的表达,发现MYL4基因在通城猪和长白猪中呈波浪式表达模式,在妊娠第33天时中外猪品种间无显著差异,但在第65和90天时长白猪中显著高于通城猪。The cDNA sequences of porcine （Sus scrofa） MYL4 gene were cloned, and verified by bioinformatics method. And its fragment was 1105 bp with an entire open reading frame （ORF）, encoding 197 amino acids. The analysis of sequence revealed that the porcine MY/At gene was highly homologous with that of other species such as canis lupus familiaris （Canis lupus familiaris） （98.4%）, Homo sapiens （Homo sapiens） （95.9%）, chimpanzee（Pan troglodytes）（95.4%） and rhesus monkey（Macaca mulatta）（95.4%） , respectively. The protein encoded by porcine MYL4 gene had conserved domain EFH and FRQ1. The expression analysis of MYL4 gene was carried out in the fetus skeletal muscle （at 33, 65 and 90 d post coitus, dpc） by real-time quantitative PCR. The results indicated that MYL4 gene was expressed with wave model in both Tongcheng and Landrace pigs. There was significant difference at 65 and 90 dpc, but no significant difference at 33 dDc between Tongcheng and Landrace.

关 键 词：猪 MYL4基因 序列分析 表达 胚胎骨骼肌 

分 类 号：S188[农业科学—农业基础科学]