瓜类细菌性果斑病菌群体感应信号分子的检测及其对致病性的影响  

Detection of Quorum Sensing Signal Molecule of Acidovorax avenae subsp,citrulli and Its Regulation on Pathogenicity

作　　者：陈涛[1] 钱国良[1] 杨小丽[2] 马俊义[2] 胡白石[1] 刘凤权[1] 

机构地区：[1]南京农业大学植物保护学院农业部病虫检测与治理重点实验室,江苏南京210095 [2]新疆农业科学院植物保护研究所,新疆乌鲁木齐860091

出　　处：《农业生物技术学报》2008年第4期653-657,共5页Journal of Agricultural Biotechnology

基　　金：国家重大基础研究前期研究专项(No．2005CCA02800)资助。

摘　　要：利用JZA1高效检测菌株生物显影技术,首次从瓜类细菌性果斑病菌(Acidovorax avenae subsp.citrulli)检测到群体感应信号分子(acyl-homoserine lactone,AHL),并证明其侧链为8个碳原子,将能够降解细菌信号分子的aiiA基因转化到瓜类细菌性果斑病菌NJF10菌株中,显著降低了其信号分子的产生。室内接种试验表明,该转化菌株的致病性明显的减弱,证明其对果斑病菌的致病性具有调控作用。The signal molecules （acyl-homoserine lactone, AHL） from melon bacterial fruit blotch （BFB） pathogen （Acidovorax avenae subsp.citrulli） was firstly detected by high efficient cells-JZA1 bioassay method and its side chain was composed of 8 carbon atoms. Gene aiiA which could degrade AHL molecules was transformed into BFB pathogen strain NJF10. The AHL production of transformed NJF10 was significantly reduced. Inoculation tests showed that pathogenicity of transformed strain was dramatically reduced. It showed that AHL produced by BFB pathogen was related to pathogenicity. This result lays a foundation for controlling melon BFB by signal interference strategy.

关 键 词：瓜类细菌性果斑病菌 群体感应 AIIA基因 信号干扰 

分 类 号：S188[农业科学—农业基础科学]