BCG-CpG-DNA对重组HBsAg免疫原性的影响  

Effect of BCG-CpG-DNA on the immunogenicity of HBsAg

作　　者：李凤祥[1] 赵爱华[1] 张洁[1] 王国治[1] 

机构地区：[1]中国药品生物制品检定所,北京100050

出　　处：《微生物学免疫学进展》2008年第3期10-14,共5页Progress In Microbiology and Immunology

基　　金：国家高技术研究发展计划(2006AA02A240)

摘　　要：为了研究BCG-CpG-DNA对重组HBsAg免疫原性的影响,了解其佐剂功能。采用不同剂量BCG-CpG-DNA与不同剂量重组(汉逊酵母)表达的HBsAg或Al-HBsAg混合免疫小鼠,与同剂量铝佐剂疫苗对比,以放射免疫法检测抗-HBs中和抗体水平和抗体持续时间,ELISA法检测抗体亚类。结果显示,BCG-CpG-DNA和HBsAg混合,抗-HBs的中和抗体水平达到或高于同剂量铝佐剂疫苗;BCG-CpG-DNA和Al-HBsAg混合,抗-HBs的中和抗体水平高于同剂量铝佐剂疫苗,并有统计学显著意义(P<0.05);添加BCG-CpG-DNA组抗体水平4周时增加不明显,到10周则显著地高于同剂量铝佐剂疫苗组,IgG2a抗体水平也高于相应的对照组。实验结果表明BCG-CpG-DNA对HBsAg有较好的免疫佐剂作用,并和AL佐剂有协同作用。The effect of BCG-CpG-DNA on the immunogenieity of HBsAg was detected in oder to understand the adjuvantieity of BCG-CpG-DNA. The mice were vaccinated s.c.with hepatitis B surface antigen or alum-adsorbed HBsAg alone and combined with BCG-CpG-DNA, then measured specific anti-HBs antibodies with radioimmunoassay （RIA）, antigen- specifec IgG isotypes were tested by ELISA, The results showed that when BCG-CpG-DNA used singly, the antibody titer is identical or higer than that of adding alum; and used with alum, the titer significandy higher ,after immunizing 10w, BCG- CpG-DNA induce higher antigen-specific IgG2a titer than those of single antigen group and alum-adsorbed antigen group. And some are statistically significant （P 〈 0.05） . BCG-CpG-DNA has good immune adjuvantity, and showed synergistic effect with Alum.

关 键 词：BCG-CPG-DNA 免疫佐剂 HBSAG 

分 类 号：R392[医药卫生—免疫学]