青藤碱对红斑狼疮患者外周血T细胞的抑制作用及机制探讨  

Suppressive effects of SIN on immunological function and cytokine production by T cells from the patients with SLE

作　　者：石韫珍[1] 孟锐[2] 李丽[2] 吴长有[2] 

机构地区：[1]广东省人民医院风湿科,广州510080 [2]中山大学基础医学院免疫学教研室教育部热带病重点实验室,广州510080

出　　处：《中国免疫学杂志》2008年第9期800-803,807,共5页Chinese Journal of Immunology

基　　金：广东省中医药局资助项目(103101);国家重点基础研究发展规划(973)基金资助项目(2007CB512404);2005年度省自然科学基金研究团队项目(05200176)

摘　　要：目的:观察青藤碱(SIN)对系统性红斑狼疮患者T细胞功能的影响,探讨其免疫抑制作用机理。方法:分离SLE患者的PBMCs,加入anti-CD3及不同浓度SIN,以ELISA和流式细胞仪方法分析青藤碱对T淋巴细胞活化和Th1/Th2细胞因子产生的影响。结果:SIN可抑制SLE患者外周血T细胞产生IFN-γ、IL-2和IL-4。SIN能够显著降低CD4+T和CD8+T细胞表面活化分子CD69和CD25的表达。结论:SIN可通过抑制T细胞的活化及细胞因子的分泌,发挥免疫抑制作用。Objective： To investigate the effects of sinomenine （SIN） on the activation and function of T-cells from SLE patients and to elucidate the mechanisms for the immunosuppressive effect of SIN.Methods：PBMCs were isolated from SLE patients and stimulated with anti- CD3 in the presence or absence of different concentrations of SIN. The cytokines were measured by ELISIA, and the activation and function of T-cells were analyzed by flow cytometry. Results： SIN could inhibit IFN-γ, IL-2 and IL-4 production of T cells in SLE patients＇ peripheral blood. In addition, SIN could significantly inhibit the expression of activation molecules CD69 and CD25 by CD4^＋ and CD8^＋ T cells. Conclusion：SIN could inhibit immune responses of SLE patients by suppressing T cells activation and cytokine secretion.

关 键 词：青藤碱 系统性红斑狼疮 T细胞 细胞因子 

分 类 号：R392[医药卫生—免疫学]