地塞米松对大鼠再生肝细胞亚精胺合成酶基因转录的影响  

Regulation of Dexamethasone on Transcription of Spermidine Synthase in Regenerating Hepatocytes in Rat

作　　者：翟心慧[1] 王志华[1] 徐存拴 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《四川动物》2008年第5期943-944,956,共3页Sichuan Journal of Zoology

基　　金：河南省科技攻关项目(0624410109);河南省动物学省级重点学科经费资助(豫教财[2001]160号)

摘　　要：采用原位杂交技术研究地塞米松对大鼠再生肝细胞亚精胺合成酶(spermidine syathase,SPDS)基因表达的影响。结果显示,部分肝切除(partial hepatectomy,PH)后,再生肝细胞mRNA表达量呈现先升高后降低的变化趋势,峰值均出现在PH后9h。PH+去肾上腺使mRNA水平升高,主要表现在PH后6～12h;地塞米松处理组SPDS基因表达量明显下降,并且随着地塞米松剂量升高,mRNA表达水平降低。结果表明,地塞米松对早期再生肝细胞SPDS基因表达具有抑制作用。The effect of dexamethasone on gene expression of spermidine synthase （SPDS） in regenerating rat hepatocytes was investigated by in situ hybridization. The results showed： in all partial hepatectomy （PH） rats, expressing level of SPDS was gradually rising, then falling with time, and the highest was at 9^th after PH. In the adrenalectomized rats with PH, SPDS mRNA level rose after PH, especially from 6th to 12th h. However, the adrenalectomized rats with PH exposured to examethasone （0.1, 0.2, and 2 mg/kg） , the expressing level of SPDS was reduced with dose-dependent manner. The results suggested that gene expression of SPDS was inhibited by dexamethasone in the regenerating rat hepatoeytes, mainly in early stage of liver regeneration.

关 键 词：大鼠 肝再生 地塞米松 亚精胺合成酶 

分 类 号：Q343[生物学—遗传学]