参附注射液中特殊色谱成分的分析  

Discrimination of the Special Chromatographic Component in Shenfu Injection

作　　者：王颖[1] 龙敏[2] 余晓琴[1] 刘仲义[1] 周娟[1] 

机构地区：[1]四川省食品药品检验所,成都市610036 [2]成都市第六人民医院,成都市610051

出　　处：《中国药房》2008年第27期2114-2116,共3页China Pharmacy

摘　　要：目的:对参附注射液水溶性成分指纹图谱中的一组特殊色谱峰的来源、产生条件、变化规律进行分析。方法:采用混合型固相萃取柱对成分进行初步分类;采用高效液相色谱-二极管阵列检测法对经酸碱处理的参附注射液及其中间体进行分离鉴定。结果:初步认为该组特殊色谱峰与在吐温存在的情况下某些具有紫外吸收的物质产生类似聚合的反应有关。结论:本试验结果有助于参附注射剂的质量控制。OBJECTIVE： To analyze the origin, producing conditions, regular pattern of the group Ⅰ special chromatographic peaks in the finger prints of the watersoluble component in Shenfu injection.METHODS： The special chromatographic component was preliminarily categorized by mixed solid phase extraction column（SPE） ; and the acid - alkali - treated Shenfu injection and its intermediates were isolated and identified by HPLC-DAD .RESULTS： It is initially assumed that the appearance of special chromatographic peak was related to the reaction similar to the polymerization reaction of the materials characteristic of ultraviolet absorption in the presence of Tween.CONCLUSION：This result obtained in this study is helpful for the quality control of Shenfu injection.

关 键 词：参附注射液 特殊色谱成分 分析 混合型回相萃取柱 高效液相色谱-二极管阵列检测法 

分 类 号：R283.611[医药卫生—中药学] R927[医药卫生—中医学]