载脂蛋白基因型多态性与妊娠期肝内胆汁淤积症相关性分析  

Association of apolipoprotein E gene polymorphism and intrahepatic cholestasis of pregnancy

作　　者：黄淑晖[1] 刘淮[1] 付群[1] 

机构地区：[1]江西省妇幼保健院产科,南昌330006

出　　处：《江西医药》2008年第8期789-792,共4页Jiangxi Medical Journal

摘　　要：目的探讨载脂蛋白基因(ApoE)多态性与妊娠期肝内胆汁淤积症(ICP)的关系。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测67例ICP孕妇与70例正常孕妇ApoE基因多态性,分析ApoE基因型和等位基因与ICP发病的关系。结果对照组ε2、ε3、ε4等位基因频率分别为10.7%、82.9%、6.4%,ApoE3/3频率为68.6%,ICP组与对照组ApoE基因型和等位基因频率差异无统计意义(P>0.05),ε4等位基因频率和ApoE3/4基因型频率在ICP组孕妇中偏高,但与对照组相比无统计意义(P>0.05)。结论ApoE基因型和等位基因频率分布在ICP组和正常孕妇组存在平衡,ApoE基因可能与ICP无关联。Objective To investigate the relationship between polymorphisms of the apolipoprotein E gene and patient with intrahepatic cholestasis of pregnancy of JiagnXi Han nationality crowd. Methods ApoE genotypes of 67 cases with ICP and 70 cases of control were detected by PCR-RFLP.ApoE genotypes and alleles frequencies were compared.Results The frequencies of the ApoEaUeles geneε2/ε3/ε4 in the control group were 10.7%, 82.9%, 6.4% respectively.The frequencies of the ApoEε3/3 genotypes were 68.6%,ApoEε3/4 genotype ande4 alleles gene were high in ICP group than in control group but have no statistical significance.Conclusion An equilib rium state between ApoE alleles and genotypes was established in both cholestatic women and controls.

关 键 词：妊娠期肝内胆汁淤积症 载脂蛋白E 基因 聚合酶链反应(PCR) 限制性片段长度多态性(RFLP) 

分 类 号：R714.255[医药卫生—妇产科学]