3种不同运输方式对红细胞悬液质量影响的实验研究  

Study of the effects of three different types of transportations on the quality of red cell suspension

作　　者：范娅涵[1] 肖瑞卿[1] 李兵[1] 蒋天伦[1] 黎儒青[1] 林武存[1] 赵树铭[1] 

机构地区：[1]中国人民解放军重庆血站第三军医大学西南医院输血科,重庆400038

出　　处：《中国输血杂志》2008年第8期577-580,共4页Chinese Journal of Blood Transfusion

摘　　要：目的探讨飞机、火车和汽车远距离运输对红细胞悬液质量的影响。方法实验分为4组,随机从经飞机、火车、汽车运输的保存了约20d的红细胞悬液中各取10U,作为3个实验组;以未经运输的库存20d的红细胞悬液10U作为对照组。用全自动生化分析仪测定LDH、Na+、K+和Cl-的浓度,用三波长比色法测定FHb,用血气分析仪检测PO2,并测定红细胞无氧糖酵解率和红细胞渗透脆性,观察各组指标间的差异;用扫描电镜观察各组红细胞的形态。结果无氧糖酵解率,飞机、火车2运输组分别为(42.9±5.67)%和(42.2±3.1)%,明显低于汽车运输组和对照组[分别为(60.4±5.9)%和(70.1±7.8)%];FHb在飞机和火车运输组中分别为(1.37±0.21)和(1.28±0.41)mg/ml,明显高于汽车运输组和对照组[(0.37±0.05)和(0.31±0.03)mg/ml];LDH含量和Na+浓度,飞机、火车2运输组明显高于汽车运输组和对照组;飞机运输组PO2明显低于其余各组,其红细胞渗透脆性也明显增加;火车运输组K+浓度明显高于其余各组;Cl-浓度则各组均无明显变化。电镜下,飞机、火车2运输组的棘形红细胞明显增多,边缘不整齐,并有聚集现象;汽车运输组的红细胞多数保持正常形态,少数红细胞呈球形或边缘不整齐;对照组红细胞形态正常呈双凹圆盘状,细胞均匀混悬。结论血液运输方式明显影响血液质量,路况良好的汽车运输方式在一定情况下更优于飞机和火车运输方式。Objective To study the effects of aircraft, railway and highway transportations on the quality of red cell suspension. Methods Red cell suspensions from aircraft, railway and highway transportations were randomly selected, 10 units each, and stored for about 20 days as test groups, Another 10 unites of red cell suspensions were collected and stored for 20 days to serve as the control group. Biochemical indicators, including lactic dehydrogenase （ LDH ）, Na＋ , K＋ and Cl - were tested by automatic biochemistry analyzer. Free hemoglobin （ FHb ） was measured by chromatometry of 3 wavelengths. PO2 was detected by blood gas analyzer. The rate of red blood anaerobic glycolysis and osmotic fragility were also tested. The microstrueturo of rod blood cells （ RBCs ） was observed by scanning electron microscope. Results The rates of anaerobic glyeolysis of aircraft and railway transported red cell suspensions were { 42. 9 ±5.6 ） % and （ 42. 2 ± 3.1 ） %, respectively, significantly lower than those of highway transportation and the control group, which were （ 60.4 ± 5.9 ） % and （ 70. 1± 7. 8 ） % , respectively. FHb concentrations in erythroeyte suspension from aircraft and railway transportations were （ 1.37 ±0. 31 ） mg/ml and （ 1.28 ±0. 41） mg/ml, respectively, significantly higher than those of the highway transportation and the control group, which were （ 0. 37 ± 0. 05 ） mg/ml and （ 0.31 ± 0. 03 ） mg/ml, respectively. LDH level and Na＋ concentrations in erythroeyte suspension from aircraft and railway transportations were significantly higher than those from highway transportation and the control group. PO2 was decreased significantly in red cell suspension from aircraft transportation, and the osmotic fragility was increased. K＋ was increased significantly in red cell suspension from railway transportation. C1- was not changed. The airoraft and railway transported RBCs showed agglutination, spherical changes and ragged edges. Many thorn RBCs could be found in these

关 键 词：血液质量 运输 长距离 

分 类 号：R331.141[医药卫生—人体生理学] R457.1[医药卫生—基础医学]