不同植物生长调节物质对杂种落叶松胚性愈伤组织增殖的影响  

Effect of Different Plant Growth Regulators on Embryogenic Callus Proliferation of Hybrid Larix

作　　者：王伟达[1] 李成浩[1] 杨静莉[1] 张含国[1] 

机构地区：[1]林木遗传育种与生物技术教育部重点实验室(东北林业大学),哈尔滨150040

出　　处：《东北林业大学学报》2008年第9期5-7,共3页Journal of Northeast Forestry University

基　　金：黑龙江省科技攻关重点项目(GB06B303-6);国家自然科学基金(30671701)项目

摘　　要：以日本落叶松×长白落叶松的胚性愈伤组织为材料,研究不同植物生长调节物质对胚性愈伤组织增殖和体细胞胚形成能力的影响。结果表明:2,4-D质量浓度对胚性愈伤组织增殖有显著影响,胚性愈伤组织增殖率随2,4-D质量浓度的升高而下降。在添加2,4-D0.1mg·L-1+BA0.05mg·L-1+KT0.05mg·L-1与NAA0.5mg·L-1+BA0.05mg.L-1+KT0.05mg.L-1的培养基中,胚性愈伤组织可以长期维持与增殖;在添加IBA0.5mg·L-1+BA0.05mg·L-1+KT0.05mg·L-1的培养基中愈伤组织的增殖效果最好,增殖系数达到4.6倍,而且转到体细胞胚成熟培养基后形成的体细胞胚数也最多,达到88.6个·g-1。在添加2,4-D0.1mg·L-1+BA0.05mg·L-1+KT0.05mg·L-1的培养基增殖的胚性愈伤组织形成的体细胞胚的萌发率和植株再生率最高,分别为66.7%和50%。The effects of various plant growth regulator combinations on proliferation and subsequent somatic embryo development of Larix leptolepis × L. olgensi embryogenic callus were investigated. The results are as follows ： 2,4 - D with different concentrations has significant influence on the proliferation of embryogenic callus. The proliferation rate declines with increasing of 2,4 - D concentrations. Medium with different combinations of plant growth regulators has remarkable effect on maintenance of embryogenic callus, and 2,4 - D 0.1 mg · L^ - 1 ＋ BA 0.05 mg· L^-1 ＋ KT 0.05 mg · L^-1 or NAA 0.5 mg · L^-1 ＋ BA 0.05 mg · L^-1 ＋ KT 0.05 mg · L-1 is suitable for embryogenic callus maintenance. IBA 0.5 mg·L^-1 ＋ BA 0.05 mg· L^ -1 ＋ KT 0.05 mg · L^ -1 was found to be best for embryogenic callus proliferation and somatic embryo development, with 4.1 times proliferation rate, and 88.6 somatic embryos from 1 g embryogenic callus. Both germination and pla- ntlet regeneration rate were highest on medium containing IBA 0.5 mg · L^-1 ＋ BA 0.05 mg · L^-1 ＋ KT 0.05 mg · L^-1 , with 66.7% and 50%. respectively.

关 键 词：日本落卉松×长白落叶松 体细胞胚发生 胚性愈伤组织 增殖 

分 类 号：S718.43[农业科学—林学]