肿节风浸膏对腮腺早期放射损伤作用的实验研究  

Experiment of Radioprotective Early Radiation Damage of Sarcandra Glabra on Salivary Gland:Observe the Tissue Morphology following fractionated Radiation on Head-neck Region in Guinea-pig Models

作　　者：秦俭[1] 滕家安[1] 王仁生[1] 周英琼[2] 

机构地区：[1]广西医科大学第一附属医院放疗科,广西南宁530007 [2]桂林医学院病理学教研室,广西桂林541001

出　　处：《中国辐射卫生》2008年第3期269-271,共3页Chinese Journal of Radiological Health

基　　金：广西自然科学基金资助项目(桂科自0542090)

摘　　要：目的研究肿节风浸膏能否保护豚鼠腮腺早期放射损伤,及放疗结束后继续给药能否促进腺体的恢复,并探讨其可能机制。方法成年雄性豚鼠50只随机分为正常对照组、药照组和单照组。药照组和单照组于照射剂量50Gy和50Gy后60d分别设置A、B两个观察组,采用γ射线常规分割局部照射的方法,并分别给予肿节风浸膏和0.9%生理盐水。观察豚鼠的体重变化、腮腺重量及组织形态学改变、残存腺体面积和炎症细胞计数。结果药照组和单照组在照射初期出现体重暂时性下降后以不同速度增长,药照组平均体重高于单照组;照后腮腺出现不同程度的腺体萎缩和间质纤维化,药照组组织形态学改变优于单照组;B组腮腺重量高于A组和正常对照组,药照B组最高(P<0.05);照后腺体面积出现不同程度减少,单照组更显著,药照B组腺体面积较药照A组增加(P<0.05),单照组则无明显改变(P>0.05);单照组炎症细胞计数显著高于药照组和正常对照组(P<0.05),药照组和正常对照组无差异(P>0.05)。结论肿节风浸膏对豚鼠腮腺早期放射损伤有一定的保护作用,照射结束后继续给药有助于腺体的修复和再生,减轻放射诱导的炎症反应可能是其保护机制之一。Objective To investigate the ability of Sarcandra glabra extracts to ameliorate the early radiation damage on salivary gland in guinea - gig models, to promote the gland recovery as continued administration after radiation, as well as analyzed the possible mechanism. Methods 50 guinea - pigs were randomly divided into normal control group, radiation control group（ radio -control） and medicine radiation group（ reed -group）. In the last 2 groups, A ,B observation groups were set up at total dose 50Gy and 60 days after 50Gy, respectively. Gamma rays and fractionated radiation delivered to head - neck region were applied, and Sarcandra glabra extracts and 0.9% normal sodium were administered to them respectively. Body weight, parotid gland weight, histopathologic change, remaining gland areas and inflammatory cell counts were observed. Results The body weight of 2 radiation groups grew by different speed after weight loss in the initial stage of radiation. Med-group performed better in body weight, histomorphology and remaining gland areas, when compared to radio - control. Parotid gland atrophy and interstitial fibrosis occurred to some extent after radiation. The gland weight of group B were higher than that groups A and normal -control group, med -group B was the highest one （P 〈 0.05 ）. The postradiation remaining gland areas declined in varying degrees. It enlarged in med - group B than in reed - group A （ P 〈 0.05 ） while there was no change between 2 radio - control groups （ P 〉 0.05 ）. The inflammatory cell counts of radio - control was higher than that of the other groups（ P 〈 0.05 ）. No differences were observed between med-groups and normal control group（ P 〉 0.05 ）. Conclusion Sarcandra glabra extracts can protect early damage induced by radiation on parotid gland in guine- pig models, and help gland recover as continued administration after radiation ended. Alleviating the inflammatory reaction induced by radiation may be one of the mechanisms of radioproteetion.

关 键 词：肿节风 腮腺 辐射防护 放射损伤 病理学 

分 类 号：R818.05[医药卫生—放射医学]