单次及重复限制应激对大鼠行为及脑内单胺类递质的影响  

Effect of single or repeated restraint stress on the behavior and the brain monoamines neurotransmitters in rats

作　　者：周建松[1] 李凌江[1] 曹霞 李卫晖[1] 李则宣[1] 

机构地区：[1]中南大学湘雅二医院精神卫生研究所精神科,长沙410011 [2]湘雅三医院健康管理中心

出　　处：《中国行为医学科学》2008年第9期772-774,共3页Chinese Journal of Behavioral Medical Science

基　　金：国家自然科学基金项目（30670751,30470621）

摘　　要：目的研究单次及重复应激对大鼠行为及部分脑区单胺类神经递质及其代谢产物的影响。方法采用限制应激模型（6h·次^-1·d^-1，重复应激连续10d）。采用空场试验、高架十字迷宫试验评估大鼠焦虑水平，高效液相色谱法检测大鼠前额叶、海马和下丘脑的单胺类神经递质。结果与对照组比较，单次应激组在空场实验中央格停留时间少（P〈0．01），在高架十字迷宫实验闭臂停留时间多（P〈0．01）、开臂停留时间少（P〈0．01）；重复应激组在中央格停留时间、在开／闭臂停留时间与对照组差异有显著性（P〉0．05）。递质分析表明，与对照组前额叶5-羟色胺[（245．84±31．32）ng／g]、5-羟吲哚乙酸[（200．69±38．47）ng／g]比较，单次应激大鼠前额叶[（314．05±31．31）ng／g，（286．32±30．10）ng／g]水平较高（P〈0．01）；与对照组海马[（182．16±16．48）ng／g，（284．13±63．86）ng／g]比较，单次应激组海马[（282．21±18．98）ng／g，（502．82±77．98）ng／g]水平较高（P〈0．01）；与对照组相比，重复应激组海马[（314．15±17．42）ng／g，（576．17±51．05）ng／g]较高（P〈0．01）。结论大鼠单次应激产生明显的应激反应，而重复暴露于相同负性事件能够导致对应激的适应；海马5-HT释放的增加可能是应激适应的神经生物学基础。Objective To investigate the effect of single or repeated restraint stress on the behavior and the brain monoamines neurotransmitters in rats. Methods The behavior were observed by the Open Field test and the Elevated Plus-Maze test, the monoamines neurotransmitters and their metabolites in homogenates obtained from the frontal cortex, hippocampus and hypothalamus were assessed by HPLC. Results The single stressed group showed more anxiety behaviors in the Open Field test and the Elevated Plus-Maze test than the control, while the repeated stressed group did not. In the single stressed group, the level of 5-HT and 5-HIAA in the frontal cortex （5-HT：314.05 ± 31.31,5-HIAA：286.32 ± 30.10） and hippocampus （5-HT：282.21 ± 18.98,5-HIAA：2.82 ± 77.98 ） were signifieantly higher than that of the control（ frontal cortex ：5-HT ：245.84 ± 31.32,5-HIAA ：200.69 ± 38.47;hippocampus：5-HT： 182. 16 ± 16.48,5-HIAA：284. 13 ± 63.86） （P 〈 0.01 ）. In the repeated stressed group, the level of 5-HT and 5-HIAA in the hippocampus was significantly higher than that of the control （P 〈 0.01 ）. Conclusion This study indicated that single stress induce the anxiety behavior,repeat exposed the same adverse event can make the rats adaptation to this kind of stress, The single and repeated stress can increase the release of the 5-HT in the hippoeampus, which may be the critical neurobiology of the stress adaptation.

关 键 词：应激 适应 心理学 神经递质 色普法 高效液相 

分 类 号：R33[医药卫生—人体生理学]