南极冰藻Chlamydomonas sp.L4的UV-B辐射效应研究  

Effect of UV-B radiation on Antarctic ice microalga Chlamydomonas sp.L4

作　　者：阚光锋[1,2] 史翠娟[2] 缪锦来[3] 李光友[3] 

机构地区：[1]中国海洋大学海洋生命学院,青岛266003 [2]哈尔滨工业大学(威海)海洋学院,威海264209 [3]国家海洋局海洋生物活性物质重点实验室,青岛266061

出　　处：《高技术通讯》2008年第9期966-970,共5页Chinese High Technology Letters

基　　金：国家自然科学基金(40506005);哈尔滨工业大学(威海)研究基金(HIT(WH)200517)资助项目

摘　　要：实验研究了从南极采集的冰藻 Chlamydomonas sp.L4经强 UV-B 辐射胁迫后的生物学效应及逆境适应性。研究显示,经过强 UV-B 胁迫,冰藻 L4细胞内迅速产生大量的活性氧,丙二醛含量升高;同时,SOD 活性快速提高,使活性氧和丙二醛含量维持在细胞容许范围之内。膜脂肪酸成分分析发现不饱和指数增加了近4倍,膜流动性增加,从而维持膜的正常功能。光学显微镜、扫描和透射电镜观察表明,UV-B 辐射使细胞体积显著增大,黏性多糖分泌增多,细胞壁增厚,未知黑色颗粒密度增加,L4可能通过上述结构变化把 UV-B 屏蔽在胞外。脂肪颗粒的增加是细胞在逆境生存的能量保障。此外,细胞中类囊体片层、线粒体和细胞核等结构基本没有变化,维持细胞基本的代谢和能量供应。The effect of ultraviolet B （UV-B） radiation on an ice microalga sample collected from the Antarctica and its adversity adaptation were experimentally studied. The study showed that under stress of strong UV-B radiation, much active oxygen generated promptly in the sample cells, which led to the accumulation of MDA. In addition, the activity of SOD increased rapidly to maintain cells in a normal metabolic status. The analysis of fatty acid composition showed that the index of unsaturated fatty acids （IUFA） in cytoplasm membrane increased by nearly 4 times, and the fluidity of membrane accelerated accordingly. The results of optical, scanning and transmission electron microscopy showed the volume of the sample increased obviously under UV-B stress. The secretion of extmcellular substances and increasing depth of cell wall shielded UV-B radiation rightly. Fat granules increased and supplied energy to cells in adversity. Additionally, the structure of thylakoid, mitochondria and cell nucleus changed slightly under UV-B radiation, which maintained the fundamental metabolism of organisms.

关 键 词：南极冰藻 UV-B辐射 膜脂过氧化 膜脂肪酸 形态结构 逆境适应性 

分 类 号：Q948[生物学—植物学]