小花盾叶薯蓣未减数花粉的人工诱导研究  

Inducement of Unreduced Pollen in Dioscorea parviflora

作　　者：董志渊[1] 郭华春[1] 

机构地区：[1]云南农业大学农学与生物技术学院薯类作物研究所,云南昆明650201

出　　处：《西部林业科学》2008年第3期6-12,共7页Journal of West China Forestry Science

基　　金：云南省科技厅项目(2002ZY-2)

摘　　要：首次进行了秋水仙素和低温处理诱导产生小花盾叶薯蓣未减数花粉的研究,为该种植物的有性多倍化育种奠定了基础。用0.1%秋水仙素处理处于减数分裂的小花盾叶薯蓣雄花花蕾24 h、48 h、72 h、96 h和对其花蕾作4℃低温处理48 h、72 h、96 h的结果表明,以0.1%秋水仙素浸泡处理72 h的效果最佳,其大花粉比例为49.20%,未减数配子比例为46.73%;经过诱导处理的花粉萌发率为26.97%,而未经处理花粉的萌发率为52.15%;所获小花盾叶薯蓣的未减数花粉在培养基上能够萌发,但其花粉管伸长速度低于正常花粉。The unreduced pollens of D. parviflora were induced by 0. 1% colchicines and low temperature for sexual polyploidization. The results showed that with the treatments of 0. 1% colchicines for 24 h, 48 h, 72 h and 96 h and 4℃ for 48 h, 72 h 96 h, 0. 1% colchicines for 72 h is the optimal treatment and produced 49.02 % huge pollen and 46.73 % unreduced gametes. The pollen germination ratio of pollen subject to the treatment of 0. 1% colchicines for 72 h is 26.97 %, comparing with 52. 15% of non-treatment pollen. Moreover, the unreduced pollen had a certain germinating ability, but the process of pollen tube growth of unreduced pollen is slower than that of the non-treatment pollen.

关 键 词：小花盾叶薯蓣 未减数花粉 人工诱导 

分 类 号：S632[农业科学—蔬菜学]