海绵来源放线菌sh6004发酵产物中的活性生物碱  

Bioactive alkaloids from sponge-derived actinomyces sh6004

作　　者：付海超[1] 赵文英[1] 钟惠民[1] 洪葵[2] 顾谦群[1] 朱伟明[1] 

机构地区：[1]教育部海洋药物重点实验室中国海洋大学医药学院 [2]中国热带农业科学院热带作物生物技术研究所,海南海口571101

出　　处：《中国海洋药物》2008年第4期14-20,共7页Chinese Journal of Marine Drugs

基　　金：海洋药物教育部重点实验室开放基金(KLMD-OUC-200607);国家863计划(2007AA09Z447)

摘　　要：目的对一株来源于胶州湾海绵的放线菌sh6004发酵产物中的抗肿瘤活性成分进行分离和鉴定。方法采用溶剂萃取、硅胶柱色谱及制备HPLC等分离手段对该菌株发酵产物的活性部位进行了活性追踪分离,通过理化性质及波谱学手段进行化学结构鉴定,以SRB法和滤纸片法分别评价了化合物的抗肿瘤和抗菌活性。结果与结论从发酵产物中分离得到4个生物碱类化合物,其结构分别鉴定为bisdethiobis(methylthio) gliotoxin(1),放线菌素D(2),放线菌素C_(2a)(3),放线菌素C_3(4)。放线菌素是该菌株的主要活性成分,在浓度为10μg·mL^(-1)时,化合物1～4表现出较强的抗金黄色葡萄球菌和抗枯草芽孢杆菌作用。Objective To isolate and identify the antitumor components of the fermentation of the actinomyces sh6004 derived from a piece of sponge （unidentified） collected in Jiaozhou Bay. Methods The antitumor components were isolated by bioassay-guided fractionation and using solvent extraction, silica gel column chromatography and preparative HPLC. Their structures were established by physieochemical properties and spectral analyses. The cytotoxicity of compound 4 and the antimicrobial activities of compounds were evaluated by SRB and paper disc method, respectively. Results and Conclusion Four alkaloid compounds were isolated and identified as bisdethiobis- （methylthio）-gliotoxin（1）, actinomycin D （2）, actinomycin C2; （3）and actinomycin C3 （4）, respectively. Actinomycins were the main antitumor components produced by actinomyces sh6004. Compounds 1-4 showed antimicrobial activities against Staphylococcus aureus and Bacillus subtilis at a concentration of 10ug· mL^-1.

关 键 词：海洋放线菌 抗菌 抗肿瘤 生物碱 放线菌素 

分 类 号：R931.711[医药卫生—生药学] Q503[医药卫生—药学]