cNHX1基因转化草莓的研究  

Research on Transformation of Strawberry with cNHX1 Gene

作　　者：魏振林[1] 董玲[2] 伊连云[2] 

机构地区：[1]德州学院生物系,山东德州253023 [2]德州学院机电系,山东德州253023

出　　处：《安徽农业科学》2008年第24期10363-10364,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]探讨将完整的含柑橘cNHX1基因转化为草莓植株。[方法]以弗吉尼亚草莓品种为试材构建了含柑橘cNHX1基因的植物表达载体,并进行了酶切鉴定,然后将表达载体导入农杆菌EHA105中,获得了工程菌株;利用农杆菌转化法,把cNHX1基因转化成草莓植株,并利用PCR方法鉴定了转化植株。[结果]利用农杆菌转化法获得了转cNHX1基因,通过进一步利用含Kam的平板进行筛选,获得了纯合的转基因植株;对获得的转基因草莓植株提取叶片DNA,利用引物F01和R02进行扩增,结果在草莓基因组中可以扩增出1.63kb的目的条带,证明cNHX1基因已经整合到草莓基因组。[结论]为获得耐盐程度显著提高的转基因草莓奠定了初步基础。[ Objective] The study aimed to discuss to translate the intact cNHX1 gene isolated from citrus into the strawberry plants. [ Method] With Fujiniya strawberry as tested material, the binary expression vector contained cNHX1 gene isolated from citrus was constructed and identified by double- enzyme digestion analysis. Then this vector was introduced into EHAlfl5 strain of Agrobacterium tumefaciens to obtain the engineering strain. By using Agrobacterimn mediated method, the cNHX1 gene was translated into the strawberry seedlings which were identified by PCR. [ Result] The cNHX1 translation gene was obtained by Agrobacterium mediated method and the homozygous transgenic plant was got by further screening by using plate with Kam. The leaf DNA was extracted from the obtained transgenic plant of strawberry and amplified by using F01 and R02 primers, as a result, the objective brand with 1.63 kb was amplified from the genome of strawberry, thus it was proved that the cNttXI gene was integrated into the genome of strawberry. [ Conclusion] This study laid the foundation for getting the transgenic plant of strawberry with much high salt tolerance.

关 键 词：cNHX1 草莓番茄 转化 

分 类 号：S668.4[农业科学—果树学]