分光光度法同时测定血浆中的诺氟沙星和氧氟沙星  

Simultaneous Detection on Norfloxacin and Ofloxacin in Plasm by Spectrophotometry

作　　者：李艳霞[1] 张恒松[1] 黄喜根[1] 张琼[1] 

机构地区：[1]江西农业大学理学院,江西南昌330045

出　　处：《安徽农业科学》2008年第25期10747-10749,共3页Journal of Anhui Agricultural Sciences

基　　金：江西省教育厅2006年度科技计划资助赣教技字[2006]144号

摘　　要：[目的]探索同时测定诺氟沙星和氧氟沙星的价廉、简便和快捷的方法。[方法]在pH值为1．81 Britton-Robinson（B-R）缓冲溶液中采用4种化学计量学方法（CLS，PCR，PLS和RBF-ANN）对诺氟沙星和氧氟沙星的合成样品光谱数据进行处理和分析，探讨各种化学计量学方法对该体系的解析能力。[结果]诺氟沙星和氧氟沙星的线性范围分别为0．6～13．8mg/L，0．5～15．0mg/L；检出限分别为0．14、0．24mg/L.试验所用方法的回收率都在95％～105％。实际样品中可能存在的300倍的Mg^2＋、葡萄糖、Zn^2＋、Ca^2＋、K^＋，10倍的柠檬酸钠、Fe^2＋、Cu^2＋均不会对诺氟沙星和氧氟沙星产生干扰。用PCR和PLS对数据进行处理的结果较好。[结论]PCR和PLS对合成样中2种药物浓度的预报结果较好，该法还被用于实际样血浆中2种药物的测定，获得了较好的定量分析结果。[ Objective ] The aim was to explore the cheap, convenient and quick method for simultaneous detection on norfloxacin and ofloxacin. [Method] The spectrum data of the synthetic samples of norfloxacin and ofloxacin was treated and analyzed by four chemometrics methods （CLS, PCR, PLS and RBF-ANN） in the pH = 1.81 Britton-Robinson （B-R） buffer solution and the analysis ability of each chemometrics method to the system was discussed. [Result] The linear range of norlloxacin and ofloxacin was 0.6- 13.8 mg/L and 0.5 - 15.0 mg/L, resp. and the detection limit was 0.14 and 0.24 mg/L, resp. The recovery rate of the methods used in the experiment was all between 95% and 105%. The ions likely existed in the practical sampies, such as Mg^2＋ with 300 times, glucose, Zn^2＋ , Ca^2 ＋ , K^ ＋ , sodium citrate with 10 times, Fe^2 ＋ and Cu^2＋ , could not produced interference on norfloxacin and ofloxacin. The effect of data treated with PCR and PLS was better. [Conclusion] The result of the 2 medicine concn, in the synthetic sample forecasted by the PCR and PLS was better and the method was used to detect the 2 medicine in the plasm of the practical sample, which obtained the better quantitative analysis result.

关 键 词：分光光度法 诺氟沙星 氧氟沙星 血浆 

分 类 号：O657.32[理学—分析化学]