两优培九抽穗期倒二·倒三叶片光合膜部分光能转化特性的研究  

Study on Characteristics of Conversion of Light Energy in Photosynthetic Membrane from Top Second Leaf and Top Third Leaf of Liangyoupeijiu during Heading Stage

作　　者：项秀兰[1] 陈国祥[1] 李小蕊[1] 高志萍[1] 施大伟[1] 韩彪[1] 江玉珍[1] 于光辉[1] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210046

出　　处：《安徽农业科学》2008年第25期10779-10782,10795,共5页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金资助项目(30671254);高等学校博士学科点专项科研基金(20060319005)

摘　　要：[目的]在光合膜水平上对两优培九倒二、倒三叶的光能转化特性进行研究,进一步探讨功能叶倒二、倒三在抽穗期的生理变化,以揭示不同衰老程度叶片的光合功能。[方法]以抽穗期的两优培九为材料,测定倒二、倒三叶的叶绿素、类胡萝卜素、可溶性蛋白和可溶性总糖含量以及类囊体膜PSⅠ、PSⅡ电子传递活性、类囊体膜室温吸收光谱、类囊体膜室温发射光谱等指标。[结果]倒二、倒三叶的叶绿素和类胡萝卜素含量随采样时间的延长而下降;可溶性蛋白含量、可溶性总糖含量都下降,但倒三叶下降幅度比倒二叶大;PSⅠ电子传递活性都在不断下降,PSⅡ电子传递活性则先上升后下降;类囊体膜室温吸收光谱都是先上升后下降,两者的室温发射光谱则是先下降后上升。[结论]功能叶倒三在抽穗期的生理变化比倒二更明显,叶片的光合功能比倒二叶弱,衰老比倒二叶快。[ Objective] Based on the level of photosynthetic membrane, characteristics of conversion of Light energy of top second leaf and top third leaf of Liangyoupeijiu were studied to explore the function of the top second leaf and top third leaf physiological changes in the heading stage, to reveal the photosynthetic function of different aging leaves. [ Method] Taking Liangyoupeijiu as material, content of chlorophyll, carotenoid, soluble protein, total soluble sugar and electron transfer activity of PSI and PSU, the absorption spectra of thylakoid membrane at room temperature and emission spectra of thylakoid membrane at room temperature of the top second leaf and the top third leaf were determined. [ Result ] The contents of chlorophyll and carotenoid of the lop second leaf and the top third leaf decreased with the extension of time sampling. Both the soluble protein content and total soluble sugar eontent decreased, but the rate of declining of the top third leaf was bigger than that of the second leaf. PSI electron transfer activity of two function leaves Were constantly dedining, and their PSⅡ electron transfer activity declined after rising. Absorption spectra of thylakoid membrane at room temperature increased at the early time, and then decreased, while the emission spectra at room temperture descended firstly and then increased. [Conclusion] The physiological changes of the top third leaf in heading were more evident than those of the top second leaf. The photosynthetic function of the top third leaf was weaker than that of the top second leaf. The aging rate of top third leaf was faster than that of the top second leaf.

关 键 词：倒二叶 倒三叶 光合色素 可溶性蛋白 可溶性糖 类囊体膜 

分 类 号：S511[农业科学—作物学]