血清浓度对TGF-β1刺激成纤维细胞增殖的影响  

The Proliferate Effect of Fibroblast with Different Concentration Serum Induced by TGF-β1

作　　者：刘苹[1] 李平[1] 周元国[1] 雷英[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所分子生物学中心,重庆400042

出　　处：《西南军医》2008年第5期1-3,共3页Journal of Military Surgeon in Southwest China

基　　金：国家重点基础研究发展规划资助项目(G1999054205);国家自然科学基金资助(30400468)

摘　　要：目的比较低血清浓度(0.4%)和正常血清浓度(10%)培养条件对TGF-β1刺激成纤维细胞增殖的影响。方法原代大鼠皮肤成纤维细胞分别采用0.4%和10%血清浓度培养,加TGF-β1刺激细胞后,分别用BrdU ELISA法检测细胞增殖情况,噻唑蓝(MTT)比色法制作细胞生长曲线。结果0.4%和10%血清浓度下,250pg/ml TGF-β1均促进成纤维细胞增殖(P<0.01),并促使生长曲线的峰值提前和增加。而25ng/ml TGF-β1均抑制成纤维细胞增殖(P<0.05),并导致生长曲线峰值的滞后和降低。结论血清浓度对大小剂量TGF-β1刺激成纤维细胞增殖变化无明显影响。Objective To compare the effects between low - concentrated FBS （ Fetal Bovine Serum） （ 0. 4 % ） and high - concentrated FBS （ 10% ） on fibroblast proliferation induced by TGF - β1 （ transforming growth factor beta - 1 ）. Methods Primary dermal fibroblasts of rats were cultured by low concentration FBS （0.4%） and high concentration FBS （10%） medium respectively; after TGF- β1 was added, MTT assay was applied to detect the fibroblast proliferation and BrdU ELISA method was applied to made out cell growth currve. Results In both low concentrated FBS {0.4%） and high concentrated.FBS （10%） condition, 250pg/ml TGF- β1 promoted cell proliferalion （ P 〈 0.01）, the peak of the cell growth curve appeared earlier and its values increased; 25ng/ml TGF- β1 inhibited cell proliferation （ P 〈 0.05 ）, and led to a later appearance of the peak of the cell growth curve and a decrease of its values. Conclusions The FBS concentration has no significant influence on the fibroblast proliferation induced by high or low dose TGF- β1.

关 键 词：血清 TGF-Β1 细胞增殖 MTT法 BRDU ELISA法 

分 类 号：R332[医药卫生—人体生理学]