金属酞菁催化ABTS氧化的研究  

Catalyzed Oxidation of ABTS by Metallophthalocyanines

作　　者：汤雁[1] 黄丹婷[2] 方华[1] 陈曌[1] 黄俊[1] 

机构地区：[1]武汉理工大学光纤传感技术与信息处理教育部重点实验室,武汉430070 [2]华中科技大学生命科学与技术学院,武汉430074

出　　处：《武汉理工大学学报》2008年第9期46-49,共4页Journal of Wuhan University of Technology

基　　金：国家自然科学基金(60537050,60377032)

摘　　要：研究了4种金属酞菁MPc(M=MnⅡ,CoⅡ,CuⅡ,NiⅡ)对2,2'-连氮-双(3-乙基苯并噻唑-6-磺酸)(ABTS)的催化作用及反应最适条件。采用分光光度计在420 nm下测定吸光度的变化来衡量其催化能力。催化反应符合一级反应动力学。4种MPc的催化活性随着M的不同而不同,其中MnPc最为显著。在磷酸氢二钠-柠檬酸缓冲液中,4种MPc催化ABTS氧化的最佳pH值均为3.0,最适催化温度均为40℃。结果表明:4种模拟酶催化性能良好,MnPc最佳。The catalyzed oxidation of ABTS by four mimics enzyme of metallo-phthalocyanines （MPc,M=Mn^Ⅱ,Co^Ⅱ,Cu^Ⅱ,Ni^Ⅱ ） and their optimum reaction condition were researched. The catalytic abilitie was tested by spectrophotometry at 420 nm with ABTS as the substrate. The catalytic oxidation of ABTS met first-grade reaction kinetics. The catalytic abilities of MPc were different with different M. The optimal pH for the oxidation of ABTS catalyzed by MPc was 3.0 for all the four MPc in 1.0 mol/L Na2HPO4-citric acid buffer solution. The optimal catalytic temperature for all the four MPc was 40 ℃ Results showed that the four mimics enzyme had good activity, especially MnPc.

关 键 词：金属酞菁 催化 模拟酶 ABTS 

分 类 号：TQ032[化学工程]