多重耐药大肠埃希菌16SrRNA甲基化酶、氨基糖苷类修饰酶基因研究  

Study on the genes of 16S rRNA methylase and aminoglycoside-modifying enzymes in multi-drug-resistant strains of Escherichia coli

作　　者：林宁[1] 孙海平[1] 糜祖煌[2] 

机构地区：[1]淮安市第一人民医院,淮安223300 [2]无锡市克隆遗传技术研究所

出　　处：《中国抗生素杂志》2008年第9期536-539,共4页Chinese Journal of Antibiotics

摘　　要：目的了解多重耐药大肠埃希菌(ECO)氨基糖苷类药物耐药机制。方法采用PCR检测20株多重耐药ECO菌11种16S rRNA甲基化酶和氨基糖苷类修饰酶基因。结果1株检出16S rRNA甲基化酶基因(5.0%),并为新亚型;16株检出氨基糖苷类修饰酶基因(80.0%)。15号株rmtB基因测得序列翻译成氨基酸序列与美国核酸库(GenBank)已登录的rmtB氨基酸不同,为新亚型。结论本组多重耐药ECO菌氨基糖苷类耐药机制与产16S rRNA甲基化酶和产氨基糖苷类修饰酶相关。多重耐药ECO菌存在新的氨基糖苷类药物耐药机制。Escherichia coli. Objective To investigate mechanism of aminoglycoside resistance in multidrug-resistant Methods The genes of 11 kinds 16S rRNA methylase and aminoglycoside-modifying enzymes were detected by polymerase chain reaction （PCR）. Results In 20 strains, 1 strain carried 16 SrRNA methylase gene （rmtB）, 16 strains were positive for the genes of aminoglycoside-modifying enzymes. There were changes of aminoacids in the sequence of RmtB of No. 15. A significant difference was found from the rest that landed in GenBank, which identified itself as new subtype. Conclusion The aminoglycoside resistance mechanism of the Escherichia coli was closely related with 16S rRNA methylase and aminoglycoside-modifying enzymes. The new mechanism of aminoglycoside resistance was discoved in multi-drugresistant strains of Escherichia coli.

关 键 词：大肠埃希菌 16S rRNA甲基化酶 氨基糖苷类修饰酶 

分 类 号：R378.21[医药卫生—病原生物学]