HPLC测定鼻咽清毒颗粒中绿原酸的含量  

Study on Standard of biyanqingdu granuies

作　　者：雷小红[1] 

机构地区：[1]湖南省怀化市药品检验所,湖南怀化418000

出　　处：《中国民族民间医药》2008年第9期32-33,共2页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:建立高效液相(HPLC)测定鼻咽清毒颗粒中绿原酸的含量。方法:色谱柱为C18(5μm,250mm×4.6mm);流动相为甲醇-0.2%磷酸(用NaOH调节pH至2.8)(20∶80);检测波长为323nm,柱温:25℃,流速:1.0mL·min-1。结果:绿原酸进样量在0.02340～0.3744ug范围内与峰面积线性关系良好,r=0.9999,平均回收率为97.9%,RSD=0.60%(n=6)。结论:该方法简便,准确,重现性好。Objective To establishes a HPLC method for chlorogenicacid of granuies Methods Uses RP-HPLC to dectect Volume was C18 （ 5μm, 250mm ×4.6mm） ,Mobilephase：methanol-0.2% phosphoric acid （ NaOH wasused to regulate pH 2.8） （20：80） ;Dectectwavelength is 323nm;The temperature of chromatopraph volume was 25℃,flow rate： 1.0ml/min. Results The ehlorogenicaci linear scope0.02340-0.3744μg （r=0.9999） ,The average recoveries were 97.98%,and the RSD was 0.60%; Conclusion The toothed was simple,accurate and reproductive.

关 键 词：高效液相色谱法 鼻咽清毒颗粒中 绿原酸 

分 类 号：R284.1[医药卫生—中药学] R944.21[医药卫生—中医学]