鸡新城疫/传染性支气管炎二联油乳剂 灭活疫苗试验研究报告(Ⅱ)——疫苗制备及其免疫效果试验  

Studies on Inactivated ND IB Oil-adjuvant Bivalent Vaccine(Ⅱ)- Vaccine Preparation and its Immunity Effects

作　　者：杨峻[1] 邵华斌[1] 王文清[1] 陈守仁[1] 黎秋华[1] 

机构地区：[1]湖北省农科院畜牧兽医研究所,武汉430209

出　　处：《湖北畜牧兽医》1997年第3期12-15,共4页Hubei Journal of Animal and Veterinary Sciences

摘　　要：根据ＮＤＶ、ＩＢＶ二各病毒混合毒种的最适稀释比例，将混合毒种接种９－１０龄鸡胚尿囊腔内（简称同胚培养），使接种鸡胚９６ｈ二种病毒毒价同时达到高峰，０．０１ｍｌ尿囊液含：ＮＤＶ≥１０＾８ＥＩＤ５０、ＩＢＶ≥１０＾７ＥＩＤ５０。收获同胚培养的胚毒，制备同胚培养的胚毒，制备油乳剂灭活疫苗。用三批疫苗分别免疫１８日龄雏鸡，免疫后４周二联疫苗的有效抗体分别为：ＮＤ血凝抑制抗体（ＨＩ）≥１：１６。NDV and IBV were mixed according to the optimal dilution proportion, then the viruses were inoculated into chicken embryos at 9 or 10-dayage. the titres of two viruses both reached the peak at 96h, the allantoic fluid contained NDV≥108EID50 and IBV ≥107EID50 per 0. 1 ml. The viruses were harvested, and the inactivated oil-adjuvant vaccines were prepared. The chickens were vaccinated at 18-day-age,ail chickens had HI titres of ND ≥1:16 and neutralizing antibody of IB≥l:8 in 4 weeks after vaccination. The duration of immunity lasted 16 weekd, and the protection rate was 8/8(ND) and 6/8(IB) respectively.

关 键 词：ND IB 疫苗 免疫效果 

分 类 号：S859.797[农业科学—临床兽医学] S858.31[农业科学—兽医学]