海洋石油烃降解细菌T_1和T_2的分子鉴定分类  

Molecular identification and classification of marine hydrocarbon degrading bacteria T_1 and T_2

作　　者：田胜艳[1,2] 王娟[1] 聂利红[1] 

机构地区：[1]天津科技大学海洋科学与工程学院,天津300457 [2]天津市海洋资源与化学重点实验室,天津300457

出　　处：《海洋科学》2008年第9期25-28,41,共5页Marine Sciences

基　　金：天津科技大学科学研究基金资助项目(20060208);天津市科技支撑计划重点项目(07ZCKFSF02000)

摘　　要：从天津滨海潮间带被石油烃严重污染的沉积物(干样含油量0.2 g/g)中,筛选分离出能够以柴油为唯一碳源生长的细菌,对其中生物量大、单株菌降解效率较高的两株细菌 T_1和 T_2进行16S rDNA 克隆,通过测定和比较16S rDNA 的部分序列对这两株细菌进行分子鉴定,以期用于石油污染的微生物修复中。结果表明,T_1与深红红螺菌(Rhodospirillum rubrum)的同源性为89%,T_2与施氏甲单孢杆菌(Pseudomonas stutzeri)的同源性为99%。Hydrocarbon degrading bacteria were obtained from oil-polluted sediments in the intertidal zone of Tianjin seashore by a soil enrichment procedure using diesel fuel as carbon source. The 16S rDNA amplified from 2 strains of these isolations, T1 and T2, which have a high biomass and a hydrocarbon degrading capability, was cloned. According to molecular criteria, nucleotides sequence of 16S rDNA was determined and analyzed. The results indicate that strain T1 is similar to Rhodospirillum rubrum （89%） and strain T2 is similar to Pseudomonas stutzeri （99%）.

关 键 词：石油烃降解细菌 16S RDNA 分子鉴定 甲单孢杆菌(Pseudomonas stutzeri) 深红红螺 菌(Rhodospirillum rubrum) 

分 类 号：Q939.1[生物学—微生物学]