海洋聚磷菌中核苷二磷酸激酶基因的克隆及序列分析  

Cloning and analyse of nucleoside diphosphate kinase gene sequence from marine polyphosphate-accumulating bacterium,Halomonas YSR-3

作　　者：任世英[1,2] 肖天[1] 

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]中国科学院研究生院,北京100049

出　　处：《海洋科学》2008年第9期61-63,共3页Marine Sciences

基　　金：国家重点基础研究发展计划项目(2006CB400604);国家自然科学基金资助项目(40376048)

摘　　要：以海洋聚磷茵 Halomonas YSR-3的总 DNA 为模板,用 PCR 法扩增核苷二磷酸激酶基因,将扩增片段克隆到 pGM-T 载体,转化 Escherichia coli TOP10菌株,经蓝白斑筛选、菌落 PCR 得到阳性克隆,测序后对序列进行 Blast 比对分析。得到的基因序列长度为420 bp,翻译后的序列与 Loktanella yestfoldensisSKA53,Jannaschia sp.CCS1,Roseobacter sp.CCS2的核苷二磷酸激酶蛋白序列相似性分别为89%,86%,85%。Nucleoside diphosphate kinase（NDPK） gene was amplified from marine polyphosphate-accumulating bacterium Halomonas YSR-3 by PCR and molecular clone techniques and inserted into a pGM-T vector. The recombinant plasmid was transformed into an Escherichia coli TOP10 strain. The positive transformants with NDPK gene were obtained by blue-white selection and clony PCR method, then the positive ones were sequenced. The sequenced fragment concluded 420 pairs of bases and was analyzed by NCBI database. The similarities of translated sequence of YSR-3 NDPK gene to Loktanella vestfoldensis SKA53, Jannaschia sp. CCS1, and Roseobacter sp. CCS2 are 89%, 86%, and 85% respectively.

关 键 词：盐单胞菌属 聚磷菌 核苷二磷酸激酶(NDPK) 

分 类 号：Q78[生物学—分子生物学]