复方五仁醇胶囊血清药化学研究(Ⅱ)  

Serum pharmacochemistry of Compound Wurenchun Capsula (Ⅱ)

作　　者：丁安伟[1] 窦志华[2] 罗琳[3] 居文政[4] 

机构地区：[1]南京中医药大学,江苏南京210029 [2]南通大学附属南通第三医院,江苏南通226006 [3]南通大学,江苏南通226006 [4]南京中医药大学附属医院,江苏南京210029

出　　处：《中草药》2008年第9期1285-1288,共4页Chinese Traditional and Herbal Drugs

基　　金：江苏省中医药局中医药科学技术研究专项课题(H05173);江苏省高校研究生科技创新计划项目(140);江苏省南通市社会发展科技计划项目(S40006)

摘　　要：目的对复方五仁醇胶囊含药血清中的药源性成分作进一步鉴定,并测定主要成分的量。方法HPLC-DAD和UPLC-MS/MS检测相结合进行成分鉴定,HPLC-UV法测定主要木脂素类的量。结果HPLC-DAD检测鉴定出含药血清中8个制剂原形成分中的7个,分别为五味子醇甲、戈米辛J、五味子醇乙、去氧五味子素、戈米辛N、五味子乙素和五味子丙素,5个代谢产物的紫外光谱图具有联苯环辛烯类木脂素的吸收特征;UPLC-MS/MS检测结果显示含药血清中除存在五味子醇甲等7个木脂素类成分外,还含有五味子酯甲;含药血清中五味子醇甲、五味子醇乙、去氧五味子素、五味子乙素的质量浓度分别为(8.1453±1.0202)、(6.6045±1.3414)、(0.5601±0.1375)、(5.9330±0.9666)μg/mL。结论复方五仁醇胶囊含药血清中的药源性成分主要有木脂素及其代谢产物。Objective To identify the drug-induced constituents in rat serum containing drug of Compound Wurenchun Capsula and determine the content of these constituents. Methods Identification of the drug-induced constituents in serum has been carried out by combinative method of HPLC-DAD and UPLC-MS/MS. The content of four lignans in serum has been detected by HPLC-LTV. Results Seven of eight original form compounds in serum have been identified as schisandrin, gomisin J, schisandrol B, deoxyschizandrin, gomisin N, schisandrin B, and schisandrin C. The UV spectrogram of five metabolites showed the absorption character of dibenzocyclooctadiene lignans. Eight lignans were identified by UPLC- MS/MS, besides schisantherin, there are seven lignan-like ones detected by HPLC-DAD. The content of schisandrin, schisandrol B, deoxyschizandrin, and schisandrin B in serum was （8. 145 3±1. 020 2）, （6. 604 5± 1. 341 4）, （0. 560 1± 0. 137 5）, and （5. 933 0± 0. 966 6） μg/mL, respectively. Conclusion Lignans and their metabolites are composed of the main drug-induced constituents in rat serum.

关 键 词：复方五仁醇胶囊 血清药化学 木脂素 

分 类 号：R284.1[医药卫生—中药学]