肉苁蓉种子的活力研究  

Study on viability of Cistanche deserticola seeds

作　　者：陈庆亮[1] 张秀省[2] 郭玉海[1] 翟志席[1] 杨重军[2] 王华磊[3] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193 [2]聊城大学农学院,山东聊城252000 [3]贵州大学农学院,贵州贵阳550025

出　　处：《中草药》2008年第9期1403-1407,共5页Chinese Traditional and Herbal Drugs

基　　金：河北省科技攻关项目(03276408D-4);重大基础研究前期研究专项(2004CCA01200)

摘　　要：目的为了改进TTC法测定肉苁蓉种子活力的方法和调查肉苁蓉种子于5℃贮藏时种子活力的变化。方法研究了TTC法中种皮、TTC溶液、次氯酸钠(NaClO)溶液和染色时间对肉苁蓉种子活力的影响,并用改进的方法测定了于5℃贮藏了不同年限种子活力。结果肉苁蓉种子于40℃下用0.5%TTC溶液染色48h后,再用0.2%NaClO溶液漂白2h后镜检;采收后贮藏于5℃保存1～2年的种子,其活力没有明显变化,但高活力种子的百分比随着贮藏年限的增加而增加。结论提供了一套方便快捷的测定肉苁蓉种子活力的方法;肉苁蓉种子于5℃贮藏有利于提高种子活力。Objective To improve the 2,3,5-triphenyl tetrazolium chloride （TTC） solution method for measuring the viability of Cistanche deserticola seeds and investigate the change in viability during storage at 5 ＂C. Methods The effect of the testa, TTC concentration, sodium hypochlorite concentration （NaClO）, and staining time were studied, and seed viability during storage at 5 ℃ was measured with the improved method. Results Seeds were kept for 48 h in 0. 5% TTC solution at 40 ℃, and then for 2 h in 0. 2% NaClO solution; Seed viability was measured under a stereomicroscope. Storing seeds of C. deserticola for 1 to 2 years at 5 ℃ had no significant effects on their viability. However, the percentage of seeds with high viability was increased with the extension of the storage time at 5 ℃. Conclusion A convenient and rapid method for measuring the viability of C. deserticola seeds is developed. Storing C. deserticola seeds at 5℃ could improve their viability.

关 键 词：寄生植物 肉苁蓉 TTC 种子活力 

分 类 号：R282.2[医药卫生—中药学]