HPLC法测定咳喘清胶囊中黄芩苷的含量  

Determination of baicalin in Ke chuan-qing capsules by HPLC

作　　者：曾莹 

机构地区：[1]福州市儿童福利院,福州350002

出　　处：《海峡药学》2008年第8期53-54,共2页Strait Pharmaceutical Journal

摘　　要：目的建立HPLC法测定咳喘清胶囊中黄芩苷的含量。方法采用HPLC法,色谱柱为Kromasil C18(4.6×250mm,5μm),检测波长为278nm,流动相为甲醇-0.1%磷酸(47∶53),流速为1.0mL·min-1。结果黄芩苷在浓度64μg^320μg范围内线性关系良好(r=0.9998),平均回收率为100.1%,RSD为1.32%。结论本方法操作简便,快速,重复性好,精密度高,结果准确可靠,可用于咳喘清胶囊中黄芩苷的含量测定。OBJECTIVE To establish a method for determination of baicalin in Ke chuan-qing cap- sules. METHODS The liquid chromatography was carried out by a Kromasil C18（4.6×250mm, 5μm）with dectection wavelength at 278nm. The mobile phase was methanol-0.1% phosphoric acid（47 ： 53）. The flow rate was 1.0mL·min^-1 .RESULTS The carlibration curve was linear in the range of 64μg- 320μg for baicalin （r = 0.9998） .The average recovery was 100.1%, RSD was 1.32% .CONCLUSION The method is rapid, quickly, accurate and reliable. It can be used to determine baicalin in Ke chuan-qing capsules.

关 键 词：HPLC法 咳哆清胶囊 黄芩苷 测定 

分 类 号：R927.2[医药卫生—药学]