Cu胁迫对大麦幼苗生长及DNA损伤效应的研究  

Plantlets Growth and DNA Damage of Barley (Hordeum Vulgare L) Under Copper Stress

作　　者：齐雪梅[1] 李培军[2] 刘宛[2] 

机构地区：[1]上海电力学院能源与环境工程学院,上海200090 [2]中国科学院沈阳应用生态研究所,辽宁沈阳100016

出　　处：《农业环境科学学报》2008年第5期1925-1928,共4页Journal of Agro-Environment Science

基　　金：上海市重点学科建设项目(P1304);国家"973"项目(2004CB418506)

摘　　要：通过石英砂培养试验,采用AFLP分子标记技术研究Cu胁迫对大麦幼苗根系基因组DNA的损伤效应,同时比较基因组DNA损伤与幼苗根系生长、可溶性蛋白含量变化对Cu污染胁迫的敏感性。结果表明,12.5￣50mg·L-1Cu处理8d后,与对照相比根长显著变短,根系内的可溶性蛋白含量显著降低;基因组DNA的AFLP图谱与对照相比发生显著变化,具体表现为谱带的消失、新谱带的出现以及谱带荧光强度的改变。三者均对低浓度的Cu污染胁迫响应敏感,其中AFLP图谱变化是对Cu胁迫响应最为敏感的生物标记物,可用于土壤Cu污染的早期诊断和生态风险评价。DNA damage effects of copper stress on the genome of barley seedlings（Hordeum vulgare L） were studied using amplified fragment length polymorphic（AFLP） technique under quartz sand culture. The sensitivity of DNA effects in the genome, root growth and total soluble protein level in root-tips to copper stress was compared. The results showed that significant inhibition of root growth and reduction of total soluble protein content in root tips of barley seedlings occurred in the range of 12.5-50 mg·L^-1 Cu after 8 d compared to that of the normal seedlings （P〈0.05）. The changes occurring in AFLP profiles of root tips following Cu treatment included loss of normal bands and appearance of new bands and variation in band intensity in comparison to that of the normal seedlings. The three above index were all sensitive to low does Cu stress, especially AFLP profiles changes and could be used as a useful biomarker for the detection of genotoxic effects of Cu pollution on organisms in soil system.

关 键 词：AFLP 生态毒性 生物标记物 

分 类 号：X503.231[环境科学与工程—环境工程]