青藤碱对肿瘤坏死因子-α诱导人脐静脉内皮细胞VCAM-1表达影响初步研究  

Effect of sinomenine on the expression of TNF-α-induced vascular cell adhesion molecule 1 on human umbilical vein endothelial cells

作　　者：黄建林[1] 吴玉琼[1] 罗敏琪[1] 魏秋静[1] 余步云[1] 古洁若[1] 

机构地区：[1]中山大学附属第三医院风湿免疫科,广州510630

出　　处：《中华风湿病学杂志》2008年第9期613-615,共3页Chinese Journal of Rheumatology

基　　金：基金项目：广东省自然科学基金资助项目（5001793）

摘　　要：目的进一步研究青藤碱（SIN）对肿瘤坏死因子（TNF）-α诱导脐静脉内皮细胞（HUVECs）血管细胞黏附分子（VCAM）-1表达的影响。方法从新鲜脐带中分离培养HUVECs。用TNF-α诱导HUVECs表达VCAM-l，实验组加入不同浓度的SIN（0．25、0．5和1．0mol／L）或地塞米松（1．0×10^-6mol／L）分别进行或联合进行干预，用流式细胞仪检测细胞表面VCAM-1表达。结果与TNF-α刺激组相比，SIN干预组细胞表面VCAM-1表达下降，抑制作用以1．0mol／L最为显著。联合地塞米松（1．0×10^-6 mol／L）进行干预，可加强SIN对TNF-α诱导的VCAM-1表达的抑制作用。结论SIN可抑制TNF-α诱导的脐静脉内皮细胞VCAM-1的表达。青藤碱与地塞米松对TNF-α诱导的VCAM-1表达的抑制有协同作用。Objective To further investigate the effect of sinomenine （SIN） on TNF-α-indueed VCAM-1 expression in human umbilical vein endothelial cells （HUVECs）. Methods HUVECs were isolated from freshly collected umbilical cords. Positive control samples were stimulated with TNF-α, but free of SIN. Negative control samples were treated in the same way, but without TNF-α and SIN. Experimental samples were cocuhured with TNF-α and SIN at various concentrations （0.25, 0.5, and 1.0 mol/L）, or TNF-α and dexamethasone （Dex） at concentration of 1.0×l0^-6 mol/L,or TNF-α with Dex （at concentration of 1.0×l0^-6 mol/L） and SIN at different concentrations （0.25, 0.5, and 1.0 mmol/L） （co-treated groups）. VCAM-1 expression was detected by flow cytometry （FCM）. Results SIN inhibited expression of VCAM-1 in TNF-α-induced HUVECs, the best effect was shown in the 1.0mmol/L SIN treated group. VCAM-1 decreased more markedly in the cotreated groups. Conclusion SIN inhibits TNF-α-induced VCAM-1 expression on HUVECs in vitro, and SIN maybe synergistic with Dex in inhibiting TNF-α-induced VCAM-1 expression on HUVECs in vitro.

关 键 词：青藤碱 血管细胞黏附分子-1 人脐静脉内皮细胞 

分 类 号：R686[医药卫生—骨科学]