基因工程技术构建预防性HPV疫苗  

Construction of Prophylactic HPV Vaccines Using Genetic Engineering Technology

作　　者：龚业莉[1] 苏冬梅[2] 刘颖[2] 夏焕章[1] 

机构地区：[1]沈阳药科大学生命科学与生物制药学院,沈阳110016 [2]沈阳三生制药有限责任公司,沈阳110027

出　　处：《中国生物工程杂志》2008年第9期130-134,共5页China Biotechnology

摘　　要：宫颈癌的发生与高危型人乳头瘤病毒(human papillomavirus,HPV)感染高度相关已得到公认。利用基因重组技术开发HPV疫苗是目前预防宫颈癌唯一有效的方法。综述了近年来在基因工程HPV疫苗研制中广泛应用的载体表达系统及其特点,依据密码子偏爱原则优化病毒外壳蛋白基因序列显著提高外壳蛋白产量等方面的研究成果,以为进一步研制与优化HPV疫苗提供参考。It＇ s commonly accepted that the infection of HPV with high-risk is the important factor in the occurrence of cervix cancer. Recombinate technologies are used to develop HPV vaccines, which is the only effective method to prevent cervix cancer so far. From the both aspects of expression vectors and codon optimization, this article reviewed the widely used expression vectors in recent years and their characteristics;As well as the research results on obviously enhancement of capsid protein by optimizing the virus capsid gene according to codon preference, which provide basis for the further manufacturing and optimization of HPV vaccines.

关 键 词：HPV 疫苗 表达系统 密码子优化 

分 类 号：Q789[生物学—分子生物学]