蜜环菌菌索多糖对四氧嘧啶致胰岛细胞损伤的保护作用  

Polysaccharide from the rhizomorph of Armillaria mella(AMP-1)protects INS-1 cells from alloxan injury

作　　者：陆军[1] 操玉平[1] 于敏[1] 沈业寿[1] 

机构地区：[1]安徽大学生命科学学院安徽省中药研究与开发重点实验室,安徽合肥230039

出　　处：《中国药理学通报》2008年第9期1160-1165,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No3040066);安徽省教育厅自然科学基金资助项目(No2003kj021;2002kj017)

摘　　要：目的研究蜜环菌菌索多糖(AMP-1)对四氧嘧啶(AXN)致胰岛细胞损伤的影响。方法培养大鼠胰岛素瘤细胞(INS-1),以AXN损伤细胞,培养液中加入不同浓度的AMP-1(2、10、50、100、500、1000mg.L-1),用MTT法测细胞存活率;使用放免法检测不同浓度AMP-1对INS-1细胞葡萄糖刺激性的胰岛素分泌量的变化;用比色法检测INS-1细胞一氧化氮(NO)、一氧化氮合酶(NOS)、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)等指标的变化。结果AMP-1可减少AXN对INS-1细胞的损伤,AMP-1处理组的INS-1细胞存活率增加;在5.6mmol.L-1、16.7mmol.L-1葡萄糖刺激下,AMP-1(50、100、500、1000mg.L-1)剂量依赖性地促进了INS-1细胞葡萄糖刺激性的胰岛素分泌;AMP-1能使INS-1细胞NOS活性降低,MDA和NO含量减少,同时增强了SOD的活性,增加了GSH的含量。结论一定浓度范围的AMP-1对葡萄糖刺激下INS-1细胞的胰岛素分泌的促进作用,可能与AMP-1能够增加细胞清除自由基能力有关。Aim To investigate the protective effects of polysaccharides from the rhizomorph of Armillaria mella （AMP-1） on alloxan injured INS-1 cells. Methods Graded concentrations of AMP-1 （2,10,50,100,500, 1 000 mg·L^-1） were added into the culture medium of alloxan injured INS-1 cells. The survival rate was measured by MTF assay. The amount of glucose-stimulated insulin secretion in different concentrations of AMP-1 was determined by radioimmunoassay （RIA）. SOD and NOS activity, NO, MDA and GSH production were assayed colorimetrically. Results AMP-1 could reduce oxidative injuries induced by alloxan in INS-1 cells. The survival rate of cells treated with AMP-1 increased significantly. In the presence of 5.6 mmol ·L^-1 or 16.7 mmol ·L^-1 glucose, AMP-1 （50,100, 500,1 000 mg·L^-1） increased glucose-induced insulin secretion in INS-1 cells in a dose-dependent manner. NOS levels and the production of NO and MDA decreased significantly by AMP-1, while SOD levels and the production of GSH increased. Conclusions AMP-1 promoted glucose-induced insulin secretion in INS-1 cells by increasing the abilities of scavenging the free radicals induced by alloxan.

关 键 词：蜜环菌菌索多糖 四氧嘧啶 大鼠胰岛素瘤细胞 胰岛素分泌 氧化损伤 

分 类 号：R284.2[医药卫生—中药学] R322.57[医药卫生—中医学]