乙型肝炎病毒感染者B淋巴母细胞系的建立  

Establishment of Immortalized B Lymphoblast Cell Line from Patients with Hepatitis B Virus Infection.

作　　者：唐祖明[1] 高健[2] 何长伦[3] 王一淳[1] 戴宇东[4] 王寿明[3] 郑纪山[2] 

机构地区：[1]东南大学生物电子学国家重点实验室,南京210000 [2]南京军区疾病预防控制中心 [3]南京军区第81医院 [4]南京市红十字血液中心

出　　处：《医学研究杂志》2008年第9期23-26,F0003,共5页Journal of Medical Research

基　　金：国家自然科学基金(30671929)

摘　　要：目的在体外建立HBV感染者永生化的B淋巴母细胞系(LCL),并鉴定其HLA-A基因亚型。方法利用EB病毒转化外周血B淋巴细胞使之永生化,用环胞菌素A(Cys A)抑制血液中T淋巴细胞的活性;用PCR-SSP法检测血样本的HLA-A基因亚型。结果成功建立了HBV感染者的永生细胞株16株,其中急性乙肝4例,慢性乙肝10例,携带者2例。所有的细胞株冻存后复苏的成功率为100%。细胞株的HLA-A基因亚型包括1101、0201、0101、2403等。结论转化后的LCL保持了良好的稳定性,既可能作为人源单抗的来源,也可作为体外研究HBV特异性免疫应答的刺激细胞和靶细胞。永生化细胞HLA-A基因亚型的鉴定有可能使其成为异体相应亚型体外特异性免疫应答的刺激细胞和靶细胞。Objective To establish immortalized B lympho - blastcell line （LCL） from patients with hepatitis B virus （HBV） infec- tion in vitro. Methods Immortalized B - lymphoblastoid cell lines were established by EB virus transformation of the peripheral blood B lymphocytes and Cyelosporin A （Cys A） restraining T cells. HLA -A gene were measured in blood mononuclear cells by PCR -SSP . Results Altogether 16 immortalized lymphoblastoid cell lines were established successfully in vitro. HLA -A alleles were detected, in- eluding 1101,0201,0101,2403,et al. Conclusion The LCLs by EB virus transformation provides a resource of target cell for further re- search on the cellular immunity of patients with HBV infection.

关 键 词：乙型肝炎病毒 EB病毒 永生化 B淋巴母细胞系 HLA—A基因亚型 

分 类 号：R512.8[医药卫生—内科学] R512.91[医药卫生—临床医学]