经前期综合征(PMS)肝气逆证大鼠下丘脑蛋白差异表达  

The Differential Expression of Hypothalamus Proteinum in PMS Liver-qi Invasion Mouse.

作　　者：孙鹏[1] 张惠云[1] 

机构地区：[1]山东中医药大学中医药经典理论教育部重点实验室,济南250355

出　　处：《医学研究杂志》2008年第9期39-42,共4页Journal of Medical Research

基　　金：国家重点基础研究发展计划项目(2006CB504804);国家自然基金项目(90209003)

摘　　要：目的研究PMS肝气逆证大鼠下丘脑蛋白质差异表达,以期为PMS肝气逆证在蛋白质水平上的发病机制寻找证据。方法采用蛋白质双向电泳技术(2-DE)对PMS肝气逆证大鼠模型下丘脑进行蛋白质图谱分析,以MALDI-TOF-MS技术进行蛋白质鉴定。结果下丘脑组织中模型组跟正常组比较:泛素羧端水解酶、清蛋白、醛缩酶蛋白表达下调;M2丙酮酸激酶、泛醇-细胞色素C还原酶核心蛋白Ⅰ、钙结合蛋白表达上调,这些蛋白质与泛素通路、中间代谢酶、代偿作用等功能有关。结论PMS肝气逆证与泛素通路、中间代谢酶、代偿作用等蛋白功能有关,提示以上6种蛋白质与PMS肝气逆证中枢发病机制关系密切。Objective To study the differential expression of hypothalamus proteinum in PMS Liver - qi invasion mouse,in order to find the evidence on pathogenesis in protein level of PMS Liver - qi invasion. Methods The spectrum of a graph on hypothalamus protein of PMS liver- qi invasion symptom was analysed by two- dimensional gel electrophoresis, protein was identified by matrix -assisted laser desorption ionization time of flight mass spectrometry（ MALDI - TOF - MS）. Results Comparing model group with normal control group in hippocampus were as follows ： Ubiquitin carboxy - terminal hydrolase L1, Albumin 1, Aldolase 3 were down - regulated; however M2 pyruvate kinase, ubiquinol - cytochrome creductase core protein I, Calbindin 2 were up - regulated,These protein were related to the func- tion of ubiquitin iter,intermediary metabolism enzyme,compensation. Conclusion PMS Liver - qi invasion was related to the proteinum function of ubiquitin iter,intermediary metabolism enzyme,compensation ,this results hint that this six protein were tight related with cen- tral pathogenesis of PMS liver - qi reversed symptom.

关 键 词：经前期综合征 肝气逆证 下丘脑 蛋白质组学 

分 类 号：R338.27[医药卫生—人体生理学] R271.11[医药卫生—基础医学]