黄瓜CsEXP10基因的电子克隆及生物信息学分析  

Silico Cloning and Bioinformatic Analysis of CsEXP10 Gene in Cucumber

作　　者：孙涌栋[1] 张传来[1] 罗未蓉[1] 张兴国[2] 

机构地区：[1]河南科技学院园林学院,新乡453003 [2]西南大学园艺园林学院,重庆400715

出　　处：《生物信息学》2008年第3期113-116,共4页Chinese Journal of Bioinformatics

基　　金：教育部优秀青年教师资助计划项目[教育司(2003)355号];河南科技学院博士科研启动项目资助(6011)

摘　　要：以黄瓜CsEXP10 cDNA片段为信息探针,利用电子克隆和序列拼接方法获得了1191bp的cDNA序列。应用生物信息学软件预测了该蛋白的理化性质、卷曲螺旋、疏水性、信号肽、跨膜结构、糖基位点、活性位点、亚细胞定位及二级和高级结构。结果表明:该蛋白是一个疏水性稳定蛋白,定位于细胞壁,含有4个α-螺旋,11个β-折叠,5个跨膜区域,10个糖基化位点,具有催化域和多糖结合域两个结构域。Using CsEXP10 cDNA fragment as probe, 1191 bp eDNA sequence was obtained by silico cloning and splicing. The character of CsEXP10 protein such as the physical and chemical properties, coiled coil, hydrophobicity, signal peptide, transmembrane domains, O- glycogylation site, active site, subcelluar localization, second and three structure were analyzed by a series of bioinformatics software. The results showed that CsEXP10 protein had four α- helix, eleven β - sheet, five transmembrane domains and ten O - glycogylation sites; it was a hydrophobie and stable protein with two functional domains, and located to cell wall.

关 键 词：黄瓜 CsEXP10基因 电子克隆 生物信息学 

分 类 号：Q543.1[生物学—生物化学]