海胆卵内生菌分离及其生物活性的初步探讨  

The Isolation and Activity Analysis of Endogenetic Bacteria from Echinus' Spawn

作　　者：张连茹[1] 杨梅[1] 于淼[1] 黄丹虹[1] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2008年第5期728-732,共5页Journal of Xiamen University：Natural Science

基　　金：国家863计划项目(2006AA09Z410);福建省自然科学基金(2006J0378)资助

摘　　要：海胆卵中含有丰富的唾液酸聚糖蛋白,作者从共生的理论出发,采用涂布法两次从海胆卵内共分离到83株菌,通过形态学的方法初步鉴定大部分为细菌.采用液体发酵法进行发酵,83株菌的发酵液均对唾液酸的特征显色反应呈阳性,唾液酸含量测定表明有1株菌唾液酸的含量达到5.8μg/mL,有7.23%的菌株唾液酸含量达到或接近3μg/mL.通过对其抗菌、抗氧化和抗肿瘤等生物活性测定表明,有8株菌具有不同程度的抗菌活性,占被检测菌株总数的19.05%;有2株菌对肺癌细胞具有明显的抑制作用.上述结果表明海洋动物海胆中含有丰富的菌种资源,具备开发生物活性物质的潜力.Echinus＇ spawn contains plenty of sialoglycoprotein. Eighty-three strains were isolated from echinus＇ spawn based on the theory of intergrowth,which presented positive result according to the special color reaction of sialic acid,and were identified as bacterium. The testing of sialie acid contents proved that one of the endophyte＇s fermentations reached 5.8μg/mL,and 7.23% of the endophytes achieved about 3μg/mL. Their bioactivities were also primarily studied. Ruselts indicated： eight strains of those endophytes had antimicrohial activities against one or more microbial, forty-two stains with antioxidant activities were screened out, and two strains of those endophyte bacteria presented obvious anti-tumor activity against zr-75-30 cell line. The results suggest that marine animal eehinus is rich of endophyte and is one of the potential resources for exploiting biological active substance.

关 键 词：海胆 内生菌 唾液酸 生物活性 

分 类 号：Q178.531[生物学—水生生物学]