外来入侵豚草天敌广聚萤叶甲的半人工饲料配方和关键因子的初步筛选  

A preliminary study on semi-artificial diets for Ophraella communa (LeSage)(Chrysomelidae:Coleoptera),a potential biological control agent of invasive Ambrosia artemisiifolia

作　　者：刘芳[1] 李保平[1] 孟玲[1] 

机构地区：[1]南京农业大学植物保护学院生物防治研究室,南京210095

出　　处：《环境昆虫学报》2008年第3期238-243,共6页Journal of Environmental Entomology

基　　金："十一五"科技支撑计划(2006BAD08A18);国家自然科学基金项目(30470295)

摘　　要：广聚萤叶甲Ophraella communa LeSage是入侵恶性豚草的天敌。参照前人对叶甲类半人工饲料的研究配方,采用19因子混水平正交试验L32(45×216),初步筛选出适合广聚萤叶甲成虫和幼虫的半人工饲料配方,筛选出影响广聚萤叶甲成虫寿命的8种关键成分:麦胚、10%KOH、葡萄糖、尼泊金、豚草叶粉、琼脂、胆固醇、大豆油;影响广聚萤叶甲2龄幼虫发育的8种关键成分是:燕麦片、琼脂、麦胚、葡萄糖、氯化胆碱、10%KOH、蒸馏水、大豆油。研究结果为进一步研制广聚萤叶甲人工饲料提供了基础。Ophraella communa LeSage （ Coleoptera ： Chrysomelidae） is a potential biological control agent of the alien invasive weed Ambrosia artemisiifolia L. （Compositae）. Based on the semi-artificial diets for other leaf beetles （Chrysomelidae）, the orthogonal experimental design was employed for 19 factors at mixed levels （L32 （4^5×2^16） ） in this study. Eight main components for adults survival were obtained, including wheat-meal, 10% KOH, dextrose, menthyl-p-hydroxybenzoate, Ambrosia plant powder, agar, cholesterol, and soybean oil. Eight main components for the 2^nd instar larval development were obtained, including roiled oats, agar, wheat meal, dextrose, choline chloride, 10% KOH, water, and soybean oil. The results form a basis for further study of the artificial diet of O. communa.

关 键 词：广聚萤叶甲 豚草 半人工饲料 正交试验 杂草生物防治 

分 类 号：Q969.9[生物学—昆虫学]