丝素—胶原复合物半月板支架负载纤维软骨细胞的实验观察  

Experimental value of culturing fibrochondrocytes using silk-collagen complex meniscal scaffold

作　　者：刘和风[1] 王俊飞[1] 黄际河[1] 秦泗通[1] 徐华[1] 蒋青[1] 

机构地区：[1]南京大学医学院附属鼓楼医院,江苏南京210008

出　　处：《山东医药》2008年第15期27-28,共2页Shandong Medical Journal

基　　金：江苏省卫生厅医学科技发展基金(H200549);江苏省自然科学基金资助项目(BK2006527);南京市医学科研重点项目(ZKX05014)

摘　　要：目的探讨蚕丝蛋白纤维(丝素)—胶原复合半月板支架作为兔纤维软骨细胞组织工程支架的可行性。方法构建丝素—胶原复合物半月板支架,将体外培养扩增的兔半月板纤维软骨细胞接种于该支架上,加入转化生长因子(TGF)-β1三维立体培养,倒置相差显微镜、扫描电镜观察细胞形态。结果丝素—胶原复合物能制成理想形状和大小的三维立体多孔半月板支架,纤维软骨细胞能良好地贴附于支架孔壁上。结论丝素—胶原复合物半月板支架细胞相容性良好,是半月板组织工程支架的可选材料。Objective To evaluate the feasibility and value of silk-collagen complex as scaffold for meniscus tissue engineering after observing how it absorbs rabbit meniscal fibrochondrocytes and affects cells characteristics. Method The artificial meniscal scaffolds were constructed with silk-collagen complex. Meniscal fibrochondrocytes were isolated and multi- plied in vitro, then fibrochondrocytes were seeded into porous silk-collagen complex scaffolds and added transfor growth factor （TGF）-β1, then cuhured in a three dimensional environment. The effects of the scaffold on cells adhesivity, proliferation and function were observed by phase-contrast microscopy, electron microscopy scanning, histological examination. Result The three-dimensional porous meniscal scaffolds could be constructed with silk-collagen complex according to meniscal size and shape. Meniscal fibrochondrocytes attached to wall of microholes of the scaffold and the compatibility was well. Conclusion Silk-collagen complex meniscal scaffold has good cellular compatibility, It may be as a selective meniscal scaffold material for building tissue engineered meniscus.

关 键 词：半月板 蚕丝 胶原 纤维软骨细胞 组织工程 

分 类 号：R684.3[医药卫生—骨科学]