各种临床类型乙型肝炎病例血清HBeAg/HBeAb和HBV DNA定量分析  

Analysis of serum HBeAg/HbeAb and HBV DNA level in hepatitis B patients of various clinical types

作　　者：雷建华[1] 梁骏[1] 杨旭[1] 贺兴鄂[1] 黄力[1] 田沂[1] 

机构地区：[1]中南大学湘雅二医院肝病研究中心,湖南长沙410011

出　　处：《中国现代医学杂志》2008年第17期2493-2495,2499,共4页China Journal of Modern Medicine

摘　　要：目的分析比较不同临床类型乙型肝炎病例的血清HBeAg/HBeAb与HBVDNA阳性率和定量水平,探讨临床病情与HBV复制水平的关系。方法收集湘雅二医院传染科在同日应用时间分辨免疫荧光法(TRFIA)和荧光定量PCR(FQ-PCR)定量测定血清HBeAg/HBeAb和HBVDNA水平的不同临床类型乙肝病例共802例,比较各组病例的HBeAg/HBeAb和HBVDNA阳性率和定量水平。结果各组病例间HBeAb水平差异无显著性,慢性轻度病例HBeAg和HBVDNA阳性率及定量水平均较高,急性或亚急性重型以及HBeAb阳性急性黄疸型病例HBeAg和HBVDNA阳性率及定量水平均较低,其他临床类型病例HBeAg和HBVDNA阳性率及定量水平差异均无显著性。结论不同临床类型乙肝病例的血清HBeAb水平差异无显著性,急性乙肝恢复期HBV复制水平较低,但慢性轻度病例HBeAg和HBVDNA较高而急性或亚急性重型病例较低,可能与机体免疫状态相关。[Objective] To compare the positive rate of the presence of HBeAg/HbeAb or HBV DNA and their average levels in serum of hepatitis B patients of various clinical types, so as to explore the relationship between clinical status and HBV replication.[Methods] Data of 802 patients inflicted with hepatitis B in the Infectious Dis- ease Department of Second Xiangya Hospital, whose serum HBeAg/HbeAb and HBV DNA level were assayed on the same day by time-resolved fluoroimmunoassay （TRFIA） and real-time fluorescence quantitative PCR （FQ- PCR） re- spectively, were collected. The positive rate of HBeAg/HbeAb or HBV DNA presence and their average levels were compared among patients of various clinical types. [Results] HBeAb level was not significantly different among vari- ous types. The positive rate of HBeAg and HBV DNA presence and their average levels were significantly higher in chronic moderate cases, while comparatively lower in acute or subacute severe cases or acute hepatitis with positive HBeAb. The positive rate of HBeAg or HBV DNA presence and their average levels did not show significant differ- ence among other types of hepatitis. [ Conclusion] HBeAb levels of various types of hepatitis B patients did not show significant difference, HBV replication level in convalescent acute hepatitis eases were lower. However, the av- erage levels of HBeAg and HBV DNA were significantly higher in chronic moderate eases, while comparatively lower in acute or subaeute severe eases. It may be related with the immunological status of the patients.

关 键 词：乙型肝炎 E抗原 E抗体 HBV DNA 

分 类 号：R512.62[医药卫生—内科学]