人绒毛膜促性腺激素调控尿道下裂兔阴茎组织血管内皮生长因子的研究  

Adjusting effect of human chorionic gonadotrophin in the vascular endothelial growth factor in rabbit phallus with hypospadias

作　　者：杨晓峰[1] 李德彪 韩雪冰[3] 戎冬文[1] 刘晶[1] 

机构地区：[1]山西医科大学第一医院泌尿外科,太原030001 [2]山西长治和平医院 [3]山西省肿瘤医院泌尿外科

出　　处：《中华泌尿外科杂志》2008年第9期643-645,共3页Chinese Journal of Urology

基　　金：山西省自然科学基金资助项目（991079）

摘　　要：目的探讨人绒毛膜促性腺激素（HCG）调节尿道下裂兔阴茎组织中血管内皮生长因子（VEGF）的作用。方法选用新西兰兔于孕期第19天时，非那雄胺片剂10mg·kg^-1·d^-1研成细粉，加蒸馏水适量灌胃连续给药4～7d。自然分娩后选择尿道下裂幼兔35只，分5组，每组7只，4组分别肌内注射HCG100、200、400、600U，1次／d，共7d；另1组仅注射等量生理盐水。另取7只正常幼兔作为正常组。药物干预后3周，取幼兔阴茎组织，测定VEGF含量。结果尿道下裂幼兔HCG100、200、400、600U肌内注射组阴茎组织VEGF含量分别为（5．00±2．37）、（5．63±1．73）、（10．35±2．34）、（16．91土2．34）pg／ml，生理盐水组为（3．99±1．19）pg／ml;正常组为（14．82土3．32）pg／ml。正常组与尿道下裂组100、200、400UHCG注射组间比较差异均有统计学意义（P〈0．05），与600UHCG注射组间比较差异无统计学意义（P〉0．05）；400U组与100、200、600UHCG注射组间比较差异均有统计学意义（P〈0．05）；100、200UHCG注射组与生理盐水注射组比较差异无统计学意义（P〉0．05）。结论幼兔阴茎组织VEGF浓度降低与尿道下裂的发生有相关性。一定剂量的外源性HCG可刺激尿道下裂幼兔阴茎组织VEGF浓度增加。Objective To study the function of human ehorionic gonadotrophin（HCG） in adjusting the vascular endothelial growth factor（VEGF） in rabbit phallus with hypospadias. Methods Rabbit hypospadias mode was made by Finasteride. After the spontaneous delivery, 35 rabbits were divided into 5 groups with 7 in each group. Four groups accepted HCG intramuscular injection for 7 consecutive days with dosages of 100,200,400 and 600 U, respectively. The control group accepted the same dosage of saline injection. Another 7 normal rabbits were used as normal controls without intervention. After 3 weeks, the rabbit phallus tissue was collected and the VEGF levels were detected by ELISA. Results Rabbits with hypospadias accepted HCG 100, 200, 400, 600 U injection had the phallus tissue VEGF level of 5.00±2.37, 5.63±1.73, 10.35±2.34 and 16.91±2.34 pg/ml, respectively. While the rabbits accepted saline injection had VEGF levels of 3.99± 1.19 pg/ml. The normal rabbit phallus tissue contained VEGF level of 14. 82± 3. 32 pg/ml. There were significant differences between normal group and the rabbits accepted HCG 100, 200, 400 U injection （P〈 0. 05）, but there was no difference with the rabbits accepted HCG 600 U injection （P〉0.05）. The VEGF level in rabbits accepted 400 U HCG injection had significant difference with rabbits accepted 100, 200, 600 U HCG （P〈0.05）. The VEGF levels in rabbits accepted 100, 200 U HCG injection had no difference with rabbits accepted saline injection（P〉0.05）. Conclusions The VEGF in rabbit phallus with hypospadias is decreased. HCG of certain dosage could increase VEGF level in rabbit phallus with hypospadias.

关 键 词：尿道下裂 血管内皮生长因子 人绒毛膜促性腺激素 

分 类 号：R695[医药卫生—泌尿科学]