基于途径分析的L-苏氨酸发酵过程优化  

Optimization of the Fermentation Progress of L-threonine by Pathway Analysis

作　　者：陈宁[1] 朱晓光[1] 徐庆阳[1] 黄金[1] 

机构地区：[1]天津市工业微生物重点实验室天津科技大学生物工程学院,天津300457

出　　处：《天津科技大学学报》2008年第3期1-4,共4页Journal of Tianjin University of Science & Technology

基　　金：国家863项目(2006AA02Z216)

摘　　要：以L-苏氨酸生产菌株TRFC为供试菌株,在拟稳态下基于途径分析对发酵过程作出理论分析,并对发酵过程进行优化.通过途径分析方法确定L-苏氨酸合成代谢途径的11种基本模型,其中模型1、3、4、6、9、11最高理论产率为86%.根据途径分析结果,提出L-苏氨酸产生菌的发酵控制策略,并进行摇瓶及发酵罐实验验证.结果表明:在添加葡萄糖酸钠发酵过程中,L-苏氨酸产量与葡萄糖为唯一碳源时相比反而降低;且充分供氧达20%时,代谢流大量涌入目的产物,L-苏氨酸产率最大.According to the pathway analysis, fermentation progress of L-threonine was eventually optimized. There are 11 basic models to be estimated. The productivity of model 1, 3, 4, 6, 9, 11 had achieved 86%. According to the result of path- way analysis, the optimized metabolic control strategy was instituted and testified through actual fermentation experiments. It was concluded that merely compared with glucose, adding sodium gluconate almost serves to decrease the productivity of L-threonine. In addition, 20% dissolved oxygen effectively allows metabolic flux into the biosynthesis pathway of L- threonine, thereby maximizing the productivity of L-threonine.

关 键 词：L-苏氨酸 途径分析 葡萄糖酸钠 代谢工程 溶氧 

分 类 号：Q93[生物学—微生物学]