三种真菌对洋地黄毒苷的生物转化特性研究  

Studies on Biotransformation Identity of Digitoxin by Three Fungi

作　　者：王丽娟[1] 王敏[1] 温竹[1] 刘增兵[1] 

机构地区：[1]天津市工业微生物重点实验室天津科技大学生物工程学院,天津300457

出　　处：《天津科技大学学报》2008年第3期8-12,共5页Journal of Tianjin University of Science & Technology

基　　金：天津市应用基础及前沿技术研究计划重点项目(08JCZDJC15200)

摘　　要：考察了新月弯孢霉(Curvularia lunata)AS3.3589、蓝色犁头霉(Absidia coerulea)CICC40302和雷斯青霉(Penicillium raistrickii)ATCC10490对洋地黄毒苷的生物转化特性.结果表明:新月弯孢霉AS3.3589转化洋地黄毒苷得到2个产物,即产物Ⅰ和产物Ⅱ,产物Ⅰ的转化率为27%,产物Ⅱ的转化率为5%;蓝色犁头霉CICC40302转化洋地黄毒苷得到1个产物,即产物Ⅲ,转化率为6%;雷斯青霉ATCC10490对洋地黄毒苷不发生转化反应.通过优化新月弯孢霉KA-91生物转化反应的工艺条件,使转化产物Ⅰ的转化率达到38%,产物Ⅱ的转化率达到10%.Biotransformed identity of digitoxin is investigated by Curvularia lunata AS3.3589, Absidia coerulea CICC40302 and Penicillium raistrickii ATCC1049. Results of the experiment are these： Two products are gotten when digi- toxin is biotransformed by Curvularia lunata AS3.3589, named product Ⅰ and product Ⅱ, and the biotransformation yields are 27% and 5%; One product is gotten when digitoxin is biotransformed by Absidia coerulea CICC40302, named product Ⅲ, and the biotransformation yield is 6%; Penicillium raistrickii ATCCI049 can not biotransform digitoxin. The optimum conditions of transformation by Curvularia lunata AS3.3589 are studied by transformated experiment. After that, the bio- transformation yield of product Ⅰ is attained to 38% and of product Ⅱ is attained to 10%.

关 键 词：洋地黄毒苷 新月弯孢霉 蓝色犁头霉 雷斯青霉 生物转化 

分 类 号：Q939.97[生物学—微生物学]