微生物基因工程育种技术的研究进展  

Progress in Study of Microbial Genetic Engineering Breeding

作　　者：代云见 王明蓉 杜天飞 

机构地区：[1]中国医药集团四川抗菌素工业研究所,成都610052

出　　处：《国外医药（抗生素分册）》2008年第5期193-196,200,共5页World Notes on Antibiotics

摘　　要：微生物基因工程育种是20世纪末发展起来的分子定向育种技术,该文重点概述了基因定点突变、易错PCR、DNA重排及基因组重排这几种微生物基因工程育种的基本原理和操作过程,并对这些方法的研究进展进行了扼要综述。Microbial genetic engineering breeding is a molecular-directed breeding technology that was developed at the end of the 20th century. This review paper mainly emphasizes on the basic principles and operation processes of microbial genetic engineering breeding including site-directed mutagenesis, error-prone PCR, DNA shuffling and genome shuffling. Additionally, the progress in the research of above-mentioned methods or technology is also summarized.

关 键 词：基因工程育种 基因定点突变 易错PCR DNA重排 基因组重排 

分 类 号：Q789[生物学—分子生物学]