老年人梅毒筛查阳性与荧光梅毒螺旋体抗体吸收法及蛋白质印迹法复测结果的对比分析  

Comparison of screening tests and confirmatory tests of fluorescent treponemal antibody absorption test and Western blotting in the elderly

作　　者：王凤超[1] 朱安友[1] 葛鑫[1] 胡建国[1] 柏平[1] 

机构地区：[1]蚌埠医学院第一附属医院检验科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2008年第5期599-601,共3页Journal of Bengbu Medical College

摘　　要：目的:比较老年人梅毒筛查阳性与确认指标之间的关系,分析各检测指标在老年梅毒诊断的临床价值。方法:用酶联免疫吸附试验及甲苯胺红试验作为梅毒筛查,对老年人阳性标本用梅毒螺旋体明胶凝集试验检测,两种方法均为阳性者用荧光梅毒螺旋体抗体吸收法(FTA-ABS)检测及蛋白质印迹法(WB)确认,并对实验结果进行统计分析。结果:对酶联免疫吸附试验筛查梅毒的76例老年人阳性标本,用梅毒螺旋体明胶凝集试验检测有72例阳性,阳性符合率94.7%;两种方法均为阳性的72例标本经FTA-ABS及WB检测确证阳性71例,阳性符合率98.6%;并且FTA-ABS检测与WB检测结果一致。结论:为了提高老年人梅毒的检出率及正确性,对梅毒筛查阳性标本,应用TPPA进行确证,有疑义者用FTA-ABS或WB进行进一步检测确证。Objective：To compare the results by screening tests and confirmatory tests, and to evaluate the methods of detecting Treponema paUidum in the elderly. Methods：The positive samples detected by screening tests with enzyme-linked immunosorbent assay and syphilis toluidine red untreated serum test were respectively confirmed by treponema pallidum particle agglutination （TPPA）, fluorescent treponemal antibody absorption test（FTA-ABS） and Western blotting（WB） ,and then the results were analyzed statistically. Results：Seventy-two of the 76 samples were detected positive by TPPA in screening tests（94.7% ,72/76）. Of the 72 positive samples by TPPA,71 were simultaneously confirmed by FTA-ABS and WB assays （98. 6% ,71/72）. Condusiom：The positive samples of Treponema pallidum detected by screening tests in the elderly must be confirmed by TPPA assay and even by FTA-ABS and WB assays if needed.

关 键 词：梅毒螺旋体 明胶凝集试验 免疫印迹法 荧光梅毒螺旋体抗体吸收法 

分 类 号：R377.1[医药卫生—病原生物学]