产ESBLs大肠埃希菌随机扩增多态性分析  

Analysis of genotyping of hospital ESBLs-producing escherichiacoli by RAPD

作　　者：许颖[1] 李晓庆[1] 江俊[1] 

机构地区：[1]成都医学院第一附属医院检验科,四川成都610500

出　　处：《四川医学》2008年第9期1116-1118,共3页Sichuan Medical Journal

基　　金：四川省教育厅基金资助项目(06Z015)

摘　　要：目的对产ESBLs菌的耐药现状进行分析研究,对耐药菌株进行基因分型流行病学研究。方法对临床分离的50株大肠埃希菌根据CLSI标准进行初筛和确证实验,对产ESBLs菌运用随机扩增多态性分析(RAPD)进行相似性分析。结果本院产ESBLs大肠埃希菌检出率为22%,氨苄西林抗生素抗性达到80%,GC含量为60%的引物适用于大肠埃希菌的随机扩增分析,11株产ESBLs菌运用RAPD技术可分为10种基因型,其中相同基因型别的2株菌来自同一科室。结论RAPD技术运用于产ESBLs菌的相似性分型具有经济、快速、可靠的特点。Objective To analysis the cmrent resistance condition of ESBLs-producing bacterium of hospital and to estimate the epidemic of resistant bacteria genotyping. Methods ESBLs-producing E. coli were confirmed by CLSI standard and the similarity of ESBLs-producing E. coli were genotyped by RAPD（Random amplified polymorphic DNA）. Results The detection rate of ESBLs-producing E. coli Was 22% and the ampicillin resistant rate was 80% .The primer which GC content was 60% was the proper one to amplify the E. coli genome. 11 ESBLs-producing E. coli could be differentiated to 10 subgenotype. Conclusion RAPD was economic, quick and credible method for epidemic analysis.

关 键 词：大肠埃希菌 超广谱Β-内酰胺酶 随机扩增多态性分析 

分 类 号：R450[医药卫生—治疗学]