运动员骨骼肌组织提取总RNA的新方法  

New method of extracting total RNA from skeletal muscle tissues of athlete

作　　者：陈兵[1] 唐量[2] 

机构地区：[1]西安体育学院科研所,陕西西安710068 [2]陕西师范大学运动生物学研究所,陕西西安710062

出　　处：《陕西师范大学学报（自然科学版）》2008年第5期68-71,共4页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：教育部长江学者和创新团队发展计划资助项目(IRT0459)

摘　　要：为了寻求一种从微量组织中高效又简便的提取骨骼肌总RNA方法,实验对常用RNA提取方法Trizol法进行了改进和简化,提取的总RNA质量进行了电泳和光密度值检测.实验结果显示,运动员微量骨骼肌组织提取的总RNA 28S、18S和5S三条带清晰可见,RT-PCR扩增的β-actin参照基因条带具有特异性,说明该方法从微量组织可有效、简便地提取骨骼肌总RNA,提取总RNA的得率和纯度均符合分子生物学进一步实验要求,为运动相关基因的分子生物学调控机制等研究提供了一定的方法参考.To find an fast and effectual method for total RNA extracted from micro skeletal muscle tissues of athlete, the traditional Trizol method for total RNA extraction was improved, and the quality of the extracted total RNA was analysed by agarose electrophoresis and optical density test. The results show that total RNA extracted by the improved method has obvious three bands of 28S, 18S and 5S. The special bands expanded by RT-PCR is consistent with β-actin genes. RNA extracted by the method can meet the demands of gene cloning and of biomolecular research in yield and purity of total RNA. The improved method has an important reference to further study the regulating mechanisms about exercise-related genes expression.

关 键 词：总RNA 提取 骨骼肌 Β-ACTIN基因 运动员 

分 类 号：G804.7[文化科学—运动人体科学]