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作 者:丁朋辉[1] 何蓓如[1] 宋喜悦[1] 胡银刚[1] 马翎健[1] 李宏斌[1] 陈世雷[1]
机构地区:[1]西北农林科技大学农学院,陕西杨凌712100
出 处:《西北农林科技大学学报(自然科学版)》2008年第9期51-57,共7页Journal of Northwest A&F University(Natural Science Edition)
基 金:陕西省科技攻关项目(2007K02-04-01)
摘 要:【目的】研究小麦温敏不育系的育性转化温度差异及其遗传机理,选育出适应范围广的温敏不育系。【方法】对带有相同T型斯卑尔脱小麦(T.spelta var.duh.)1Bs染色体上的温敏主效基因、同一轮回亲本3314回交选育的YS型小麦温敏不育系3个姊妹系A731、A732和A733进行分期播种和分期剪穗试验,结合试验期间气象资料,比较其育性转换临界温度及可育条件下的自交结实率。【结果】A731、A732和A733的育性转换临界温度分别为18.92,17.90和18.54℃;3个姊妹系剪穗再生分蘖穗在27.85℃的可育条件下,自交结实率分别为9.80%,34.30%和14.75%,而当温度下降到24.45℃时自交结实率相应降低到5.00%,10.01%和8.35%。【结论】3个姊妹系在不同可育条件下的自交结实率有所不同,但与各自的育性转换临界温度高低无关,带有同一温敏主效基因的3个姊妹不育系的温敏特性存在差异,揭示了YS型小麦温敏不育系育性转换遗传机制的复杂性。[Objective] The study investigated the critical temperature difference of fertility exchange of thermo-sensitive male-sterile wheat lines and their genetics mechanism,and bred the thermo-sensitive male sterile lines that could be used in wide-range areas. [Method] Experiments were done to compare the ther- mo-sensitivity of three sisters lines of YS type thermo-sensitive male-sterile wheat lines A731, A732 and A733,which were bred by backcrossing with the same recurrent parent 3314 and transferred the same major thermo-sensitive gene form 1B chromosome of T. spelta vat. duh. , through sowing at different time and redeveloping tillers of the cuttillers at different time and combining their fertility exchang critical temperatures and rate of selfed seed under fertility condition with mteorologic conditions during the experiments. [Result] It showed that the fertility exchang critical temperature of A731, A732 and A733 was 18. 92, 17.90 and 18.54℃ respectively. Selfed seed rates of ratooning of 3 sister lines were 9.80%,34.30%and 14.75%under fertility condition 27.85℃ respectively. However,when the temperature descended to 24.45℃, their selfed seed rates fell to 5.00%, 10.01% and 8.35 % respectively. When under different fertile con-ditions there were different fertility-restoration levels, which was irrelevant with their fertility exchange critical temperature value. [Conclusion] Thermo-sensitive of three sister lines, which contained the same major thermo-sensitive gene,was different,indicating that the genetic mechanism of selfed seed rate of YS-type thermo-sensitive male-sterile wheat line was quite complicated.
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