蒲黄总黄酮对3T3-L1脂肪细胞过氧化物酶体增生物激活受体家族mRNA基因表达的影响  

Effects of Pollen Typhae total flavones on mRNA expressions of peroxisome proliferator-activated receptors in 3T3-L1 adipocytes

作　　者：何燕铭[1] 王文健[1] 陈伟华[1] 应健[1] 

机构地区：[1]复旦大学中西医结合研究所,上海200040

出　　处：《中西医结合学报》2008年第9期939-941,共3页Journal of Chinese Integrative Medicine

基　　金：教育部985重点工程资助项目(No2001-13);国家教委高校博士点基金资助项目(No20050246041)

摘　　要：目的:观察蒲黄总黄酮(Pollen Typhaetotal flavones,PTF)对脂肪细胞糖脂代谢和过氧化物酶体增生物激活受体(peroxisome proliferator-activated receptor,PPAR)家族基因表达的影响,并分析其改善胰岛素抵抗的可能机制。方法:蒲黄总黄酮干预3T3-L1脂肪细胞,实时定量荧光多聚酶联反应检测脂肪细胞分化相关基因PPAR家族,包括PPARα、PPARγ和PPARβ/δ mRNA的表达。结果:蒲黄总黄酮可上调PPARα、PPARγ mRNA的表达,下调PPARβ/δ mRNA的表达,与正常对照组比较,差异有统计学意义(P<0.01)。结论:蒲黄总黄酮可能通过提高3T3-L1脂肪细胞PPARα和PPARγ mRNA的表达改善胰岛素抵抗。Objective： To explore the effects of Pollen Typhae total flavones （PTF） on the mRNA expressions of peroxisome proliferator-activated receptors （PPARs） α, γ, β/δ so as to analyze its possible mechanism in improving the insulin sensitivity of 3T3-L1 adipocytes. Methods： Adipocytes were treated with PTF, and expressions of PPARα, PPARγ, PPARβ/δ mRNAs relating to the adipocyte glucose and lipid metabolism were determined by reverse transcription polymerase chain reaction. Results： PTF obviously up-regulated the expressions of PPARα and PPARγ/ mRNAs, all showing significant differences as compared with those in the normal control group （P〈0. 01）. Conclusion, With its function as an insulin sensitizer, PTF may enhance the PPARα and PPARγ mRNA expressions in 3T3-L1 adipocytes.

关 键 词：脂肪细胞 蒲黄总黄酮 游离脂肪酸 PPAR家族 

分 类 号：R285.5[医药卫生—中药学]