楸树腋芽增殖快繁技术研究  

Techniques for Tissue culture and Rapid Propagation of Catalpa bungei

作　　者：杨燕[1] 彭方仁[1] 岑显超[1] 江荣翠[1] 

机构地区：[1]南京林业大学森林资源与环境学院,南京210037

出　　处：《林业科技开发》2008年第5期65-68,共4页China Forestry Science and Technology

基　　金："十一五"国家科技支撑计划课题"楸树珍贵用材林培育关键研究与示范"(编号:2006BAD24B08);江苏省高技术项目"楸树优良无性系选育及苗木标准化生产技术研究"(编号:BG2006319)

摘　　要：选用楸树茎段为外植体进行组培腋芽增殖途径研究,结果表明:(1)利于楸树生长的最适宜基本培养基为N6培养基;(2)最佳灭菌方法:取带腋芽茎段,剪成1～2cm,自来水冲洗2h→切剥成0.5cm大小的生长点→超净台上70%酒精30 S→无菌水冲洗4次→0.1%升汞浸6min→无菌水冲洗4次一接种;(3)初代培养最适培养基:N6+6-BA1.0mg/L+NAA0.01mg/L;继代培养最适培养基:N6+6-BA2.0mg/L+NAA 0.1mg/L+Vc100mg/L+稀土2mg/L;生根培养最适培养基:N6+NAA1.0mg/L。The stem segments with axillary buds of Catalpa bungei were chosen as explants to study its rapid propagation techniques in this paper. The results were as follows ： （ 1 ） The best basic medium was N6 ; （2） The effective procedure was： The one-year-old stem segments were chose and cut into 1 -2 cm sections with one auxiliary, bud each, then washing in tap water for 2h → cutting into 0. 5 cm growing points →soaking in 70% alcohol soaking for 30s → washing in sterile water for 4 times →soaking in 0. 1% HgC12 solution for 6 min → washing in sterile water for 4 times again→ culture in medium; （3） The most suitable medium for primary culture was： N6＋6-BA 1.0 mg/L＋NAA 0.01 mg/L; The most suit- able medium for sub culture was： N6＋6-BA 2.0mg/L＋NAA0. 1 mg/L＋Vc 100 mg/L＋Lanthanide 2 mg/L; The most suitable medium for rooting induction was N6＋NAA1.0 mg/L.

关 键 词：楸树 腋芽 组织培养 快速繁殖 

分 类 号：S792.117[农业科学—林木遗传育种] S682.33[农业科学—林学]